|
| Homogenizer Application Samples
| |
|
|
| MATERIAL:
| Acetate fiber
|
| VOLUME OF MATERIAL:
| 1 g
|
| MEDIUM:
| Methanol and Freon solvent
|
| VOLUME OF MEDIUM:
| 100 ml
|
| PROCESS:
| Homogenization
|
| PREVIOUS OR CURRENT METHOD:
| Oester blender
|
| EQUIPMENT USED:
| PRO250 Homogenizer (w/ stand) or PRO300 Homogenizer with blade and sealed chamber assembly
|
| APPLICATION AND RESULTS:
| Obtained needed effect in 10 seconds, 1/6 the time of the blender.
|
|
|
| MATERIAL:
| 5 ml
|
| VOLUME OF MATERIAL:
| Aribidopsis seedlings
|
| MEDIUM:
| Extraction buffer
|
| VOLUME OF MEDIUM:
| 150 ml
|
| PROCESS:
| RNA extraction
|
| PREVIOUS OR CURRENT METHOD:
| Brinkmannn polytron
|
| EQUIPMENT USED:
| PRO200 Homogenizer with 5mm generator
|
| APPLICATION AND RESULTS:
| Excellent homogenization of entire seedling after
grinding with mortar and pestle. Got large yields from the PRO200 Homogenizer and
acceptable RNA samples.
|
|
|
| MATERIAL:
| Bacteria
|
| VOLUME OF MATERIAL:
| 500ml
|
| MEDIUM:
| Culture
|
| VOLUME OF MEDIUM:
| Not reported
|
| PROCESS:
| Complete dispersion with low foaming
|
| PREVIOUS OR CURRENT METHOD:
| Brinkmannn Polytron
|
| EQUIPMENT USED:
| PRO200 Homogenizer with 20mm generator and sealed chamber
|
| APPLICATION AND RESULTS:
| Worked perfectly with minimal foaming.
|
|
|
| MATERIAL:
| Bacterial phage-filaments
|
| VOLUME OF MATERIAL:
| .015-.02 ml
|
| MEDIUM:
| Aqueous buffer
|
| VOLUME OF MEDIUM:
| 1-1.5 ml
|
| PROCESS:
| disruption of the filament
|
| PREVIOUS OR CURRENT METHOD:
| Mechanical Shear and Hand-press
|
| EQUIPMENT USED:
| PRO200 Homogenizer with 5mm generator
|
| APPLICATION AND RESULTS:
| Achieved desired results in 25-30 sec.
|
|
|
| MATERIAL:
| Biological tissue
|
| VOLUME OF MATERIAL:
| 5ml
|
| MEDIUM:
| Not reported
|
| VOLUME OF MEDIUM:
| Not reported
|
| PROCESS:
| Break down tissue for analysis and testing
|
| PREVIOUS OR CURRENT METHOD:
| Hand mixer
|
| EQUIPMENT USED:
| PRO200 Homogenizer with 7mm generator
|
| APPLICATION AND RESULTS:
| Achieved desired results
|
|
|
| MATERIAL:
| Bovine thyroid tissue
|
| VOLUME OF MATERIAL:
| 50ml
|
| MEDIUM:
| Not reported
|
| VOLUME OF MEDIUM:
| Not reported
|
| PROCESS:
| Homogenization
|
| PREVIOUS OR CURRENT METHOD:
| Not reported
|
| EQUIPMENT USED:
| PRO200 Homogenizer with 10mm generator
|
| APPLICATION AND RESULTS:
| Worked better than expected.
|
|
|
| MATERIAL:
| Cell membrane
|
| VOLUME OF MATERIAL:
| 50 ml
|
| MEDIUM:
| PBS type
|
| VOLUME OF MEDIUM:
| 50 ml
|
| PROCESS:
| Cell membrane preparation
|
| PREVIOUS OR CURRENT METHOD:
| Large homogenizers
|
| EQUIPMENT USED:
| PRO200 Homogenizer with 5mm generator
|
| APPLICATION AND RESULTS:
| Quicker and easier than other homogenizers along with achieving same results.
|
|
|
| MATERIAL:
| Cells
|
| VOLUME OF MATERIAL:
| .5 ml
|
| MEDIUM:
|
Not reported |
| VOLUME OF MEDIUM:
|
5 ml |
| PROCESS:
|
Break open cell membranes |
| PREVIOUS OR CURRENT METHOD:
|
Freezing and thawing |
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm generator
|
| APPLICATION AND RESULTS:
|
Completely homogenized and broken down in
fifteen seconds.
|
|
|
| MATERIAL:
|
Chicken cartilage
|
| VOLUME OF MATERIAL:
|
Not reported |
| MEDIUM:
|
Sucrose
|
| VOLUME OF MEDIUM:
|
1/2 ml |
| PROCESS:
|
Homogenization without destroying the sub-cellular
components
|
| PREVIOUS OR CURRENT METHOD:
|
Dounce homogenizer |
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm generator |
| APPLICATION AND RESULTS:
|
Ran well and kept sub-cellular components
|
|
|
| MATERIAL:
|
Chicken gizzards
|
| VOLUME OF MATERIAL:
|
50g
|
| MEDIUM:
|
Acetone
|
| VOLUME OF MEDIUM:
|
200 ml |
| PROCESS:
|
Homogenization for chemical analysis |
| PREVIOUS OR CURRENT METHOD:
|
Polytron, Tissuemizer |
| EQUIPMENT USED:
|
PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with blade and
chamber assembly as well as 20mm
generator |
| APPLICATION AND RESULTS:
|
Blade system did not completely
homogenize the sample, but results were
adequate enough. However,
the 20mm generator completely homogenized the
sample in two
minutes after pre-chopping the gizzard into 1 cm
pieces.
RECOMMENDATIONS: Cutting large chunks of tissue into pieces
will
allow a greater number of applications with the
generators.
|
|
|
| MATERIAL:
|
Chlorophilia
|
| VOLUME OF MATERIAL:
|
5-10 ml
|
| MEDIUM:
|
Not reported |
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Water quality testing
|
| PREVIOUS OR CURRENT METHOD:
|
Ultrasonic
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator
|
| APPLICATION AND RESULTS:
|
Worked in homogenizing the sample |
|
|
| MATERIAL:
|
Citrus bark |
| VOLUME OF MATERIAL:
|
5 ml |
| MEDIUM:
|
Phosphate buffer |
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Extraction
|
| PREVIOUS OR CURRENT METHOD:
|
Liquid nitrogen and break up with hammer
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm or 10mm generators |
| APPLICATION AND RESULTS:
|
Able to achieve desired results with both
generator sizes.
|
|
|
| MATERIAL:
|
Clumped white cell agluts
|
| VOLUME OF MATERIAL:
|
Not reported
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Steinbach homogenizer
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm or 10mm generators
|
| APPLICATION AND RESULTS:
|
Worked well with both
generator sizes.
|
|
|
| MATERIAL:
|
Cotton roots
|
| VOLUME OF MATERIAL:
|
5-6 ml |
| MEDIUM:
|
SPW
|
| VOLUME OF MEDIUM:
|
5-6 ml |
| PROCESS:
|
Reduce in size and release hormones |
| PREVIOUS OR CURRENT METHOD:
|
Not reported
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator |
| APPLICATION AND RESULTS:
|
Worked well with tender roots, but not as
effective with hardened stems. For
best results use a tube a
little larger in diameter than the generator |
|
|
| MATERIAL:
|
Cranberries
|
| VOLUME OF MATERIAL:
|
30 ml
|
| MEDIUM:
|
Water
|
| VOLUME OF MEDIUM:
|
20 ml
|
| PROCESS:
|
Homogenization of berries in robot assembly line |
| PREVIOUS OR CURRENT METHOD:
|
None |
| EQUIPMENT USED:
|
PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with blade and sealed
chamber assembly
|
| APPLICATION AND RESULTS:
|
Worked well
|
|
|
| MATERIAL:
|
Cultured bone marrow macrophage cells |
| VOLUME OF MATERIAL:
|
Not reported |
| MEDIUM:
|
Phosphate buffered saline |
| VOLUME OF MEDIUM:
|
2.25 ml
|
| PROCESS:
|
Membrane suspension from whole cell suspension
|
| PREVIOUS OR CURRENT METHOD:
|
Ultrasound, barrel and pestle
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with l0mm generator |
| APPLICATION AND RESULTS:
|
With old system, only able to achieve 400
rpms of binding above the blank
value of 100. With the PRO200 Homogenizer
system, achieved 4000 rpms within seconds.
However, results were
too good for the experiment that was being conducted. |
|
|
| MATERIAL:
|
Drosophilia melanogaster
|
| VOLUME OF MATERIAL:
|
3-10 fruit flies |
| MEDIUM:
|
Aqueous buffer
|
| VOLUME OF MEDIUM:
|
100-300 ml |
| PROCESS:
|
Complete homogenization and release of DNA for
polymerize chain-reaction |
| PREVIOUS OR CURRENT METHOD:
|
Grinding with glass rod or
applicator sticks
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm generator |
| APPLICATION AND RESULTS:
|
Worked well in grinding the fruit flies
and preservation of DNA, however,
scientists were concerned over
the mixing of DNA molecules from different
strains as material
stuck to the generators during
processing.
RECOMMENDATIONS: Generators can be completely immersed
and
sterilized between applications with minimal disassembly. |
|
|
| MATERIAL:
|
E. Coli cells |
| VOLUME OF MATERIAL:
|
25 ml
|
| MEDIUM:
|
Not reported |
| VOLUME OF MEDIUM:
|
Not reported |
| PROCESS:
|
Breakage of cell (100%)
|
| PREVIOUS OR CURRENT METHOD:
|
Ultrasonic homogenizer
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm generator |
| APPLICATION AND RESULTS:
|
Did not get 100% breakage in 15 seconds.
Sometimes longer running times are needed to
obtain complete homogenization.
Fifteen seconds is not quite long
enough for the type of disruption needed
for this application.
|
|
|
| MATERIAL:
|
Escherichia coli |
| VOLUME OF MATERIAL:
|
Not reported |
| MEDIUM:
|
Chemical solution
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Isolation of fimbriae |
| PREVIOUS OR CURRENT METHOD:
|
None
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 20mm generator |
| APPLICATION AND RESULTS:
|
The 20mm generator produced the required
homogenization result.
|
|
|
| MATERIAL:
|
Fig Newton cookies
|
| VOLUME OF MATERIAL:
|
20 grams
|
| MEDIUM:
|
None |
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Grinding to measure moisture |
| PREVIOUS OR CURRENT METHOD:
|
Waring blender |
| EQUIPMENT USED:
|
PRO250 Homogenizer (w/ stand) or PRO300D with sealed chamber |
| APPLICATION AND RESULTS:
|
Able to grind the Fig Newton, but lack of
medium caused the paste within the
cookie to become heated and
reduces the moisture content, which had
comparable results to the
blender. A medium is recommended.
|
|
|
| MATERIAL:
|
Fish tissue
|
| VOLUME OF MATERIAL:
|
10-400 ml
|
| MEDIUM:
|
Water
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenize for future application |
| PREVIOUS OR CURRENT METHOD:
|
Hand and ultrasonic homogenizer
|
| EQUIPMENT USED:
|
PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with sealed
chamber assembly
|
| APPLICATION AND RESULTS:
|
Worked well and sealed system proved to
be essential success of
application.
|
|
|
| MATERIAL:
|
Food additives
|
| VOLUME OF MATERIAL:
|
various
|
| MEDIUM:
|
: 1) Mayonnaise 2) Salad dressing 1 gallon (PRO200 Homogenizer Process)
|
| VOLUME OF MEDIUM:
|
1 gallon
|
| PROCESS:
|
Mix food product while adding various additives in liquid
and powder form to
obtain vertical flow.
|
| PREVIOUS OR CURRENT METHOD:
|
Waring blender
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 20mm generator and PRO300 D Homogenizer with a
blade and sealed chamber.
|
| APPLICATION AND RESULTS:
|
The PRO200 Homogenizer and the 20mm generator were
unable to mix the mayonnaise and the
secret additive, however it
worked superbly on the salad dressing. The PRO300 D Homogenizer and the blade
system were used on the mayonnaise and began to mix but
there was
no vertical flow of material.
RECOMMENDATIONS: Recommend that a larger diameter generator and
PRO250 Homogenizer or PRO300 D Homogenizer be used to cause vertical flow of the material.
|
|
|
| MATERIAL:
|
Food samples |
| VOLUME OF MATERIAL:
|
1 ml
|
| MEDIUM:
|
Organic aqueous |
| VOLUME OF MEDIUM:
|
5 ml
|
| PROCESS:
|
Complete dispersion |
| PREVIOUS OR CURRENT METHOD:
|
None
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm or 10mm generator |
| APPLICATION AND RESULTS:
|
Good homogenization results
|
|
|
| MATERIAL:
|
Frog embryos
|
| VOLUME OF MATERIAL:
|
Not reported
|
| MEDIUM:
|
varied
|
| VOLUME OF MEDIUM:
|
1.5 ml
|
| PROCESS:
|
Cell disruption |
| PREVIOUS OR CURRENT METHOD:
|
Mortar and pestle
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm or 10mm generators
|
| APPLICATION AND RESULTS:
|
Worked well.
|
|
|
| MATERIAL:
|
50 ml
|
| VOLUME OF MATERIAL:
|
Not reported |
| MEDIUM:
|
Not reported |
| VOLUME OF MEDIUM:
|
50 ml |
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Not reported |
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator
|
| APPLICATION AND RESULTS:
|
Worked well. |
|
|
| MATERIAL:
|
Gypsy Moth eggs
|
| VOLUME OF MATERIAL:
|
05 ml |
| MEDIUM:
|
Aqueous
|
| VOLUME OF MEDIUM:
|
1 ml
|
| PROCESS:
|
Rupture of eggs
|
| PREVIOUS OR CURRENT METHOD:
|
Manual
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm generator |
| APPLICATION AND RESULTS:
|
Successfully ruptured eggs for further
analysis.
|
|
|
| MATERIAL:
|
Hair shampoo
|
| VOLUME OF MATERIAL:
|
15000-20000g
|
| MEDIUM:
|
None
|
| VOLUME OF MEDIUM:
|
None
|
| PROCESS:
|
Mix shampoo to desired consistency |
| PREVIOUS OR CURRENT METHOD:
|
Large mixer with 24" rotor and
4" blade
|
| EQUIPMENT USED:
|
PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with a blade and
sealed chamber assembly
|
| APPLICATION AND RESULTS:
|
The blade moved a great deal of the
shampoo because the high rpms.
|
|
|
| MATERIAL:
|
Hamster brain
|
| VOLUME OF MATERIAL:
|
10 ml
|
| MEDIUM:
|
Not reported |
| VOLUME OF MEDIUM:
|
Not reported |
| PROCESS:
|
Homogenization for RNA and protein purification
analysis
|
| PREVIOUS OR CURRENT METHOD:
|
Not reported
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator |
| APPLICATION AND RESULTS:
|
Successful completion of application.
|
|
|
| MATERIAL:
|
Hamster tissue
|
| VOLUME OF MATERIAL:
|
9 grams |
| MEDIUM:
|
Aqueous
|
| VOLUME OF MEDIUM:
|
1-5 ml
|
| PROCESS:
|
Release bacteria from tissue |
| PREVIOUS OR CURRENT METHOD:
|
None |
| EQUIPMENT USED:
|
PRO200 Homogenizer with a 10mm generator |
| APPLICATION AND RESULTS:
|
Required results achieved easily.
|
|
|
| MATERIAL:
|
Heart cells
|
| VOLUME OF MATERIAL:
|
Not reported
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Isolation of metabolites |
| PREVIOUS OR CURRENT METHOD:
|
None
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm generator
|
| APPLICATION AND RESULTS:
|
Worked well. Intended results achieved
within seconds.
|
|
|
| MATERIAL:
|
HIV Infected H9 cells |
| VOLUME OF MATERIAL:
|
20-200 g
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
50-400 ml |
| PROCESS:
|
Disrupt cell membrane |
| PREVIOUS OR CURRENT METHOD:
|
Dounce Homogenizer
|
| EQUIPMENT USED:
|
PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with blade and sealed
chamber assembly
|
| APPLICATION AND RESULTS:
|
Worked well and sealed system is a
must. However, sharpened blades may pose a
huge health risk if
poked into skin.
RECOMMENDATIONS: As an added safety
feature for working with a
such material, we would suggest the use of a
generator system
(smooth bottomed) that has no exposed sharp edges that can
break
the skin of its user.
|
|
|
| MATERIAL:
|
Human liver
|
| VOLUME OF MATERIAL:
|
100-200 ml
|
| MEDIUM:
|
Water
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Complete homogenization |
| PREVIOUS OR CURRENT METHOD:
|
None
|
| EQUIPMENT USED:
|
PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with 1) blade and
sealed chamber assembly 2)
20mm generator |
| APPLICATION AND RESULTS:
|
Worked best with the blade and sealed
chamber assembly. Chunks were too big
for the 20mm generator.
|
|
|
| MATERIAL:
|
Human tissue
|
| VOLUME OF MATERIAL:
|
5 g
|
| MEDIUM:
|
Not reported |
| VOLUME OF MEDIUM:
|
Not reported |
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Brinkmannn homogenizer
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator
|
| APPLICATION AND RESULTS:
|
Homogenized tissue well and was quieter
than previous methods.
|
|
|
| MATERIAL:
|
Human tissue |
| VOLUME OF MATERIAL:
|
.05 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenize RNA
|
| PREVIOUS OR CURRENT METHOD:
|
Not reported |
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm generator
|
| APPLICATION AND RESULTS:
|
Worked well
|
|
|
| MATERIAL:
|
Human tissue
|
| VOLUME OF MATERIAL:
|
8 ml |
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
RNA extraction |
| PREVIOUS OR CURRENT METHOD:
|
Brinkmannn Polytron |
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm generators |
| APPLICATION AND RESULTS:
|
Liked low noise level of units. Achieved
desired results.
|
|
|
| MATERIAL:
|
Human tissue
|
| VOLUME OF MATERIAL:
|
20 ml |
| MEDIUM:
|
Phosphate buffer
|
| VOLUME OF MEDIUM:
|
50 ml
|
| PROCESS:
|
Complete homogenization |
| PREVIOUS OR CURRENT METHOD:
|
shift blender, mortar and pestle |
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generators |
| APPLICATION AND RESULTS:
|
Worked very well and desired results were
achieved.
|
|
|
| MATERIAL:
|
Leaves
|
| VOLUME OF MATERIAL:
|
25 ml
|
| MEDIUM:
|
Organic buffer |
| VOLUME OF MEDIUM:
|
75 ml
|
| PROCESS:
|
Grinding of leaves for protein analysis
|
| PREVIOUS OR CURRENT METHOD:
|
Brinkmannn Polytron |
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator |
| APPLICATION AND RESULTS:
|
Good results.
|
|
|
| MATERIAL:
|
Liver cells from rat
|
| VOLUME OF MATERIAL:
|
10 ml
|
| MEDIUM:
|
Krebs-Ringer buffer |
| VOLUME OF MEDIUM:
|
20 ml
|
| PROCESS:
|
Rupturing of hepatocytes and leaving of sub-cellular
components intact |
| PREVIOUS OR CURRENT METHOD:
|
Dounce homogenizer |
| EQUIPMENT USED:
|
PRO200 Homogenizer with 1Omm generator |
| APPLICATION AND RESULTS:
|
The PRO200 Homogenizer ruptured the cells in a very
short time (2 minutes) leaving the
sub-cellular components
intact. There was minimal foaming and the compactness
and noise
level of the instrument were added benefits |
|
|
| MATERIAL:
|
Lymphocyte, brain, and thymus
|
| VOLUME OF MATERIAL:
|
.5-5 ml
|
| MEDIUM:
|
GITC buffer |
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Isolation of RNA and DNA
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and pestle, blender |
| EQUIPMENT USED:
|
PRO200 with 5mm or 7mn generators |
| APPLICATION AND RESULTS:
|
Application successful
with either generator between the volumes
of
material.
|
|
|
| MATERIAL:
|
Mice lungs
|
| MEDIUM:
|
50 ml
|
| VOLUME OF MEDIUM:
|
100 ml
|
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Virtis
|
| EQUIPMENT USED:
|
PRO200 with 10mm generator
|
| APPLICATION AND RESULTS:
|
Heating was a concern, but none resulted
and the experiment was completed
successfully.
|
|
|
| MATERIAL:
| Mosquitoes
|
| VOLUME OF MATERIAL:
| 50 mosquitoes |
| MEDIUM:
| Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Virus analysis in mosquitoes
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and pestle
|
| EQUIPMENT USED:
| PRO200 with 5mm generator |
| APPLICATION AND RESULTS:
|
PRO200 saves considerable amount of time
and works well in this
application.
|
|
|
| MATERIAL:
|
Mouse ears
|
| VOLUME OF MATERIAL:
|
1-5 ml
|
| MEDIUM:
|
Buffer solution
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Cell disruption
|
| PREVIOUS OR CURRENT METHOD:
|
Brinkmannn Polytron
|
| EQUIPMENT USED:
|
PRO200 with 5mm or 7mm generators
|
| APPLICATION AND RESULTS:
|
Cell disruption and adequate sample size
achieved with both generator sizes
(generator diameter depended on
material volume), which had considerably less
noise than Polytron.
|
|
|
| MATERIAL:
|
Mouse muscle
|
| VOLUME OF MATERIAL:
|
2 ml
|
| MEDIUM:
|
Body fluids
|
| VOLUME OF MEDIUM:
|
4 ml
|
| PROCESS:
|
Not reported
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and Pestle
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm generator
|
| APPLICATION AND RESULTS:
|
Results were acceptable
|
|
|
| MATERIAL:
|
Mouse organs
|
| VOLUME OF MATERIAL:
|
10 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Stirring motor with grinder
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with a 10mm generator
|
| APPLICATION AND RESULTS:
|
The PRO200 Homogenizer successfully completed the
application.
|
|
|
| MATERIAL:
|
Muscle brain connective tissue
|
| VOLUME OF MATERIAL:
|
50ml
|
| MEDIUM:
|
Water
|
| VOLUME OF MEDIUM:
|
100 ml
|
| PROCESS:
|
Pulverize or powder frozen muscle tissue
|
| PREVIOUS OR CURRENT METHOD:
|
Tekmar Tissu-mizer
|
| EQUIPMENT USED:
|
PRO250 Homogenizer (w/ stand) or PRO300D a 20mm generator
|
| APPLICATION AND RESULTS:
|
Generator was able to provide successful
results.
|
|
|
| MATERIAL:
|
Muscle tissue
|
| VOLUME OF MATERIAL:
|
<.5 ml
|
| MEDIUM:
|
Aqueous buffer
|
| VOLUME OF MEDIUM:
|
2 ml
|
| PROCESS:
|
Extraction
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and pestle after freezing
sample with liquid nitrogen
|
| EQUIPMENT USED:
|
PRO200 with 5mm or 7mm generators
|
| APPLICATION AND RESULTS:
|
Both generators work within an
Eppendorf tube. Both generators produce the
same good
results, because of small volume of material. Emulsion
will
exists in larger samples.
|
|
|
| MATERIAL:
|
Mustard and pepper buds
|
| VOLUME OF MATERIAL:
|
20 total buds
|
| MEDIUM:
|
Buffer
|
| VOLUME OF MEDIUM:
|
30 ml
|
| PROCESS:
|
Release of microspores and removal of supernatant
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and pestle
|
| EQUIPMENT USED:
|
PRO200 with l0nm generator
|
| APPLICATION AND RESULTS:
|
Dispersion was more than adequate.
|
|
|
| MATERIAL:
|
Nerve segments
|
| VOLUME OF MATERIAL:
|
.1 ml (5mm gen.) and 75 ml (10mm gen.)
|
| MEDIUM:
|
SDS buffer
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
| Homogenization |
| PREVIOUS OR CURRENT METHOD:
| Manual grinding and glass-glass
microhomogenizer
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm and 10mm generators
|
| APPLICATION AND RESULTS:
| Application successful.
|
|
|
| MATERIAL:
|
Nerve tissue
|
| VOLUME OF MATERIAL:
|
.1 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Wheaton mortar and pestle
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm generator
|
| APPLICATION AND RESULTS:
|
Tissue was too stringy and offered no
resistance to the
blade.
RECOMMENDATIONS: In situations where the material is too
flexible
or stringy, liquid nitrogen can be used to freeze the sample.
The
blade will then be able to cut through the frozen sample. The
liquid
nitrogen allows for the sample to be grinded very finely. A
7mm saw tooth
bottom generator would also be recommended, when
liquid nitrogen is not
available.
|
|
|
| MATERIAL:
|
Nuts
|
| VOLUME OF MATERIAL:
|
1 g
|
| MEDIUM:
|
Water
|
| VOLUME OF MEDIUM:
|
20 ml
|
| PROCESS:
|
Homogenization to recover enzymes
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and pestle and then vortex
stirrer
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator
|
| APPLICATION AND RESULTS:
|
Nuts were ground successfully.
|
|
|
| MATERIAL:
|
Ovarian tissue
|
| VOLUME OF MATERIAL:
|
10 ml
|
| MEDIUM:
|
Phosphate buffer
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
RNA and mitochondria isolation
|
| PREVIOUS OR CURRENT METHOD:
|
bounce homogenizer, Brinkmannn
Polytron
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm generator
|
| APPLICATION AND RESULTS:
|
Very good for homogenizing small amounts
of tissue. Broke up 75% of cells in
defined protocol.
|
|
|
| MATERIAL:
|
Pancreas virus tissue
|
| VOLUME OF MATERIAL:
|
1 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Extraction
|
| PREVIOUS OR CURRENT METHOD:
|
Not reported
|
| EQUIPMENT USED:
|
PRO200 with 5mm
|
| APPLICATION AND RESULTS:
|
The PRO200 worked excellent
|
|
|
| MATERIAL:
|
Peanuts and Mung beans
|
| VOLUME OF MATERIAL:
|
50 ml
|
| MEDIUM:
|
Aqueous
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenization of hypocotyls and seeds to isolate protein
|
| PREVIOUS OR CURRENT METHOD:
|
Brinkmannn Polytron
|
| EQUIPMENT USED:
|
PRO200 with 10mm generator
|
| APPLICATION AND RESULTS:
|
Dispersal achieved successfully for the
protein analysis.
|
|
|
| MATERIAL:
|
Phycomyces
|
| VOLUME OF MATERIAL:
|
1 g
|
| MEDIUM:
|
Chloroform, methanol and hydrochloric acid
|
| VOLUME OF MEDIUM:
|
10 ml
|
| PROCESS:
|
Homogenization of fibers and shear cells
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and pestle
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm generator
|
| APPLICATION AND RESULTS:
|
Worked well, but clogged occasionally.
However, this is not a major
problem.
|
|
|
| MATERIAL:
|
Placenta tissue
|
| VOLUME OF MATERIAL:
|
100 g
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
2 L
|
| PROCESS:
|
Complete homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Tekmar Tissue-Mizer
|
| EQUIPMENT USED:
|
PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with 20mm generator
|
| APPLICATION AND RESULTS:
|
Complete homogenization in 15 seconds.
|
|
|
| MATERIAL:
|
Plant tissue
|
| VOLUME OF MATERIAL:
|
50 g
|
| MEDIUM:
|
GTC
|
| VOLUME OF MEDIUM:
|
100 ml
|
| PROCESS:
|
Homogenization and RNA extraction
|
| PREVIOUS OR CURRENT METHOD:
|
Brinkmannn Polytron
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 20mm generators
|
| APPLICATION AND RESULTS:
|
Worked nicely.
|
|
|
| MATERIAL:
|
Plant tissue
|
| VOLUME OF MATERIAL:
|
10 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Freeze with liquid nitrogen and break
up with more and pestle
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm generators
|
| APPLICATION AND RESULTS:
|
Successful homogenization
|
|
|
| MATERIAL:
|
Powder
|
| VOLUME OF MATERIAL:
|
1 ml
|
| MEDIUM:
|
Aqueous
|
| VOLUME OF MEDIUM:
|
Not Reported
|
| PROCESS:
|
Homogenization of reconstituted polymers
|
| PREVIOUS OR CURRENT METHOD:
|
Hand pestle
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm generator
|
| APPLICATION AND RESULTS:
|
PRO200 Homogenizer did the job successfully.
|
|
|
| MATERIAL:
|
Powdered aluminum
|
| VOLUME OF MATERIAL:
|
.5 ml
|
| MEDIUM:
|
None
|
| VOLUME OF MEDIUM:
|
None
|
| PROCESS:
|
Dispersal but no disruption of particle shape or size
|
| PREVIOUS OR CURRENT METHOD:
|
None
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm generator
|
| APPLICATION AND RESULTS:
|
Good application.
|
|
|
| MATERIAL:
|
Quail liver
|
| VOLUME OF MATERIAL:
|
.05 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Grinding
|
| PREVIOUS OR CURRENT METHOD:
|
Freeze with liquid nitrogen and grind
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm generator
|
| APPLICATION AND RESULTS:
|
Worked well.
|
|
|
| MATERIAL:
|
Rat aorta
|
| VOLUME OF MATERIAL:
|
Not reported
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Break down rat aorta to recover as much RNA as possible
|
| PREVIOUS OR CURRENT METHOD:
|
Brinkmannn polytron after frozen, and
broken with liquid nitrogen.
|
| EQUIPMENT USED:
|
PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with 10mm generator
|
| APPLICATION AND RESULTS:
|
PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer worked successfully and results were better than
previous method.
|
|
|
| MATERIAL:
|
Rat bowel
|
| VOLUME OF MATERIAL:
|
10 g
|
| MEDIUM:
|
TCA
|
| VOLUME OF MEDIUM:
|
Not Reported
|
| PROCESS:
|
Homogenous suspension needed for membrane assays
|
| PREVIOUS OR CURRENT METHOD:
|
Brinkmannn Polytron
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator
|
| APPLICATION AND RESULTS:
|
Worked well and its ease of use were
beneficial in conducting the
experiment.
|
|
|
| MATERIAL:
|
Rat brain
|
| VOLUME OF MATERIAL:
|
25ml
|
| MEDIUM:
|
PDY buffer
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Teflon-glass homogenizer
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with an l0mm generator
|
| APPLICATION AND RESULTS:
|
Worked well and cut time drastically.
|
|
|
| MATERIAL:
|
Rat brain, liver, muscle, peripheral nerves
|
| VOLUME OF MATERIAL:
|
50 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Protein isolation
|
| PREVIOUS OR CURRENT METHOD:
|
Dounce homogenizer
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator
|
| APPLICATION AND RESULTS:
|
Worked very well.
|
|
|
| MATERIAL:
|
Rat brains
|
| VOLUME OF MATERIAL:
|
10 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
DNA extraction
|
| PREVIOUS OR CURRENT METHOD:
|
Hand mixer
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm generator
|
| APPLICATION AND RESULTS:
|
PRO200 Homogenizer achieved desired results and even crushed up the skull that was
included in the sample.
|
|
|
| MATERIAL:
|
Rat epidymous
|
| VOLUME OF MATERIAL:
|
3 ml
|
| MEDIUM:
|
SPW
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Disruption of tissue and release of sperm
|
| PREVIOUS OR CURRENT METHOD:
|
Forceps and scapel
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm and 10mm generators
|
| APPLICATION AND RESULTS:
|
7mm too small but best results were
attained with 10mm generator. Total
disruption was achieved.
|
|
|
| MATERIAL:
|
Rat jaw and teeth
|
| VOLUME OF MATERIAL:
|
.05 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and pestle
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm generator
|
| APPLICATION AND RESULTS:
|
Completely homogenized sample.
|
|
|
| MATERIAL:
|
Rat leg muscles
|
| VOLUME OF MATERIAL:
|
5 ml (7mm gen.) and 20 ml (10mm gen.)
|
| MEDIUM:
|
BSA
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and pestle
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm and 10mm generators
|
| APPLICATION AND RESULTS:
|
Ran very well. Saw teeth on 7mm and 10mm
were perfect in homogenizing the
sample.
|
|
|
| MATERIAL:
|
Rat spinal cord and root ganglia
|
| VOLUME OF MATERIAL:
|
.5 ml
|
| MEDIUM:
|
Lithium chloride
|
| VOLUME OF MEDIUM:
|
1 ml
|
| PROCESS:
|
Complete homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and Pestle
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm generator
|
| APPLICATION AND RESULTS:
|
PRO200 worked well on the sample.
|
|
|
| MATERIAL:
|
Rat tongue and tails
|
| VOLUME OF MATERIAL:
|
1 ml
|
| MEDIUM:
|
ECTA, DDT
|
| VOLUME OF MEDIUM:
|
1.5 ml
|
| PROCESS:
|
Isolate DNA protein
|
| PREVIOUS OR CURRENT METHOD:
|
Grind in blender and Mortar and pestle
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm generator
|
| APPLICATION AND RESULTS:
|
Successfully isolated the DNA protein
from the tongue and tails.
|
|
|
| MATERIAL:
|
Rat uteri
|
| VOLUME OF MATERIAL:
|
50 ml
|
| MEDIUM:
|
Water
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and pestle, Brinkmannn Polytron
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator
|
| APPLICATION AND RESULTS:
|
Did a better job than the Polytron, and
it produced less noise.
|
|
|
| MATERIAL:
|
Raw sewage
|
| VOLUME OF MATERIAL:
|
5ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Analysis of raw sewage
|
| PREVIOUS OR CURRENT METHOD:
|
Not reported
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm generator
|
| APPLICATION AND RESULTS:
|
Works well
|
|
|
| MATERIAL:
|
Rice and barley seeds
|
| VOLUME OF MATERIAL:
|
10 ml (both 7mm and 10mm gen. Test)
|
| MEDIUM:
|
Organic solvents
|
| VOLUME OF MEDIUM:
|
Not Reported
|
| PROCESS:
|
Complete dispersal of starch material
|
| PREVIOUS OR CURRENT METHOD:
|
Liquid nitrogen then mortar and pestle
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm and 10mm gener
|
| APPLICATION AND RESULTS:
|
Best results with 10mm saw tooth.
|
|
|
| MATERIAL:
|
Rodent embryo
|
| VOLUME OF MATERIAL:
|
2 ml (All gen. were tested)
|
| MEDIUM:
|
Aqueous
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenization for PCR work and DNA synthesis
|
| PREVIOUS OR CURRENT METHOD:
|
Hand pestel
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5nm, 7mm, and 1Omm generators
|
| APPLICATION AND RESULTS:
|
Excellent results achieved.
|
|
|
| MATERIAL:
|
Salmon sperm
|
| VOLUME OF MATERIAL:
|
5ml (7mm gen.) and 25 ml (20mm gen.)
|
| MEDIUM:
|
Blocking solution
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Liquefaction of sperm for DNA analysis
|
| PREVIOUS OR CURRENT METHOD:
|
23-guage-liquefaction needle
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm and 10mm generators
|
| APPLICATION AND RESULTS:
|
Desired liquefaction results in 2-3
minutes.
|
|
|
| MATERIAL:
|
Silica gel
|
| VOLUME OF MATERIAL:
|
400 ml
|
| MEDIUM:
|
Aqueous
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Dispersion
|
| PREVIOUS OR CURRENT METHOD:
|
Blender
|
| EQUIPMENT USED:
|
PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with 20mm generator
|
| APPLICATION AND RESULTS:
|
successful.
|
|
|
| MATERIAL:
|
Silicone
|
| VOLUME OF MATERIAL:
|
5 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Not reported
|
| PREVIOUS OR CURRENT METHOD:
|
Not reported
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm generator
|
| APPLICATION AND RESULTS:
|
The PRO200 Homogenizer with 7mm generator produced
excellent results.
|
|
|
| MATERIAL:
|
Skeletal muscle, fat, and liver powder
|
| VOLUME OF MATERIAL:
|
1 g
|
| MEDIUM:
|
GTC buffer or phosphate buffer
|
| VOLUME OF MEDIUM:
|
10 ml
|
| PROCESS:
|
RNA extraction through homogenized suspension
|
| PREVIOUS OR CURRENT METHOD:
|
Brinkmannn Polytron
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm generator
|
| APPLICATION AND RESULTS:
|
Worked very well and saved time and
effort.
|
|
|
| MATERIAL:
|
Skin fibroblasts
|
| VOLUME OF MATERIAL:
|
5 ml (5mm gen.) 50 ml (10mm gen.)
|
| MEDIUM:
|
Salt buffer
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Complete homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
None
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm and 10mm generators
|
| APPLICATION AND RESULTS:
|
Achieved desired results successfully
|
|
|
| MATERIAL:
|
Soil
|
| VOLUME OF MATERIAL:
|
200 ml
|
| MEDIUM:
|
None
|
| VOLUME OF MEDIUM:
|
None
|
| PROCESS:
|
Homogenization of dry soil
|
| PREVIOUS OR CURRENT METHOD:
|
Meat grinder
|
| EQUIPMENT USED:
|
PRO250 Homogenizer (w/ stand) or PRO300 D homogenizer with blade and sealed
chamber assembly
|
| APPLICATION AND RESULTS:
|
Sample ground up into fine powder
|
|
|
| MATERIAL:
|
Soybean cotyledon
|
| VOLUME OF MATERIAL:
|
100 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Grinding
|
| PREVIOUS OR CURRENT METHOD:
|
Blender
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator
|
| APPLICATION AND RESULTS:
|
Worked well.
|
|
|
| MATERIAL:
|
Soybean cotyledons
|
| VOLUME OF MATERIAL:
|
50 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and pestle
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator
|
| APPLICATION AND RESULTS:
|
Complete homogenization
|
|
|
| MATERIAL:
|
Spleen
|
| VOLUME OF MATERIAL:
|
10 ml
|
| MEDIUM:
|
Buffer
|
| VOLUME OF MEDIUM:
|
40 ml
|
| PROCESS:
|
Spleen disruption
|
| PREVIOUS OR CURRENT METHOD:
|
Tekmar Tissue-Mizer
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator
|
| APPLICATION AND RESULTS:
|
Good results.
|
|
|
| MATERIAL:
|
Spore strips
|
| VOLUME OF MATERIAL:
|
1 strip
|
| MEDIUM:
|
Tryptic Soy broth
|
| VOLUME OF MEDIUM:
|
20 ml
|
| PROCESS:
|
Break up to recover bacteria
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and pestle and vortex stirrer
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator
|
| APPLICATION AND RESULTS:
|
Broken up easily. Increased cell
disruption achieve through longer
homogenization.
|
|
|
| MATERIAL:
|
Sturgeon barbles
|
| VOLUME OF MATERIAL:
|
4 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Mitochondria extraction
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and pestle
|
| EQUIPMENT USED:
|
PRO200 with 7mm generator
|
| APPLICATION AND RESULTS:
|
7mm saw tooth achieved desired results in
five minutes. To make sure that
generated heat would not harm
sample, used ice bath.
|
|
|
| MATERIAL:
|
Tissue
|
| VOLUME OF MATERIAL:
|
.25 ml
|
| MEDIUM:
|
GTI
|
| VOLUME OF MEDIUM:
|
.5 ml
|
| PROCESS:
|
Homogenize tissue to isolate RNA
|
| PREVIOUS OR CURRENT METHOD:
| Brinkmannn instruments |
| EQUIPMENT USED:
| PRO200 Homogenizer with 7mm generator |
| APPLICATION AND RESULTS:
|
Results were extremely successful
|
|
|
| MATERIAL:
|
Tissue
|
| VOLUME OF MATERIAL:
|
10 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
25 ml
|
| PROCESS:
|
Homogenize tissue with no degradation of RNA
|
| PREVIOUS OR CURRENT METHOD:
|
Not reported
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator
|
| APPLICATION AND RESULTS:
|
Excellent results
|
|
|
| MATERIAL:
|
Tumor
|
| VOLUME OF MATERIAL:
|
Not reported
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Not reported
|
| PREVIOUS OR CURRENT METHOD:
|
Break down tumor without disrupting cells
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm generator
|
| APPLICATION AND RESULTS:
|
Successfully broke down the tumor without
disrupting cells.
|
|
|
| MATERIAL:
|
Vanilla Callus tissue culture
|
| VOLUME OF MATERIAL:
|
1/8"-1/4"
|
| MEDIUM:
|
Water
|
| VOLUME OF MEDIUM:
|
2-10 ml
|
| PROCESS:
|
Complete homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and pestle
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm generator
|
| APPLICATION AND RESULTS:
|
Successfully broke the sample down with
the 7mm generator.
|
|
|
| MATERIAL:
|
Various creams
|
| VOLUME OF MATERIAL:
|
100-250 ml
|
| MEDIUM:
|
None
|
| VOLUME OF MEDIUM:
|
250-500 ml
|
| PROCESS:
|
Emulsion with even disbursement
|
| PREVIOUS OR CURRENT METHOD:
|
Brinkmannn, IKA, and other various
mixers
|
| EQUIPMENT USED:
|
PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with a blade and
sealed chamber assembly
|
| APPLICATION AND RESULTS:
|
Complete emulsification and disbursement
with no lumps through blade system.
Replaces other methods as best
application.
|
|
|
| MATERIAL:
|
Various eye parts
|
| VOLUME OF MATERIAL:
|
5 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Break down tissue to extract cells
|
| PREVIOUS OR CURRENT METHOD:
|
None
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm generator
|
| APPLICATION AND RESULTS:
|
Worked well
|
|
|
| MATERIAL:
|
Soybean plant
|
| VOLUME OF MATERIAL:
|
5 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Rupture of samples
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and pestle
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm and 10mm generators
|
| APPLICATION AND RESULTS:
|
Successful application
|
|
|
| MATERIAL:
|
Virus
|
| VOLUME OF MATERIAL:
|
25 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Extraction of virus for electrophoresis assay
|
| PREVIOUS OR CURRENT METHOD:
|
Hand turned mixer
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator
|
| APPLICATION AND RESULTS:
|
Excellent homogenization of sample.
|
|
|
| MATERIAL:
|
Waste bacteria
|
| VOLUME OF MATERIAL:
|
.05 ml (5mm gen.) and 50 ml (10mm gen.)
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Break down of bacteria through homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Not reported
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm and 10mm generators
|
| APPLICATION AND RESULTS:
|
Products proved to be successful
|
|
|
| MATERIAL:
|
Wheat leaves
|
| VOLUME OF MATERIAL:
|
25 ml
|
| MEDIUM:
|
Extraction buffer
|
| VOLUME OF MEDIUM:
|
25 ml
|
| PROCESS:
|
Fine grinding of plant tissue for DNA analysis
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and pestle after freezing
with liquid nitrogen
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator
|
| APPLICATION AND RESULTS:
|
DNA was broken down into short pieces
that could not analyze. Big chunks were
needed for proper examination.
RECOMMENDATIONS: Perhaps a blade system can
give the larger sample sizes that are needed for this type of
application.
|
|
|
| MATERIAL:
|
Whole cornea
|
| VOLUME OF MATERIAL:
|
5 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Mortar and pestle
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm and 10mm generator
|
| APPLICATION AND RESULTS:
|
The 7mm generator was too small to handle
large cornea chunks. However, the
10mm was perfect for the
application.
|
|
|
| MATERIAL:
|
Wild tomato vines
|
| VOLUME OF MATERIAL:
|
3g (5mm gen.) and 6 g (7mm gen.)
|
| MEDIUM:
|
Aqueous buffer
|
| VOLUME OF MEDIUM:
|
5 ml (5mm gen.) and 10 ml (7mm gen.)
|
| PROCESS:
|
Dispersion and homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Virtishear mixer
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm and 10mm generators
|
| APPLICATION AND RESULTS:
|
Worked excellent.
|
|
|
| MATERIAL:
|
Yeast cells
|
| VOLUME OF MATERIAL:
|
2 ml
|
| MEDIUM:
|
Water
|
| VOLUME OF MEDIUM:
|
2 ml
|
| PROCESS:
|
Break down yeast cells
|
| PREVIOUS OR CURRENT METHOD:
|
Glass beads and Braun system
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm generator
|
| APPLICATION AND RESULTS:
|
Tried using a 7mm generator with glass
beads added. The beads were ground up
but the yeast cells were not broken up enough. A 10mm generator will probably
break the yeast cells apart enough.
|
|
|
| MATERIAL:
|
Sea Weed
|
| VOLUME OF MATERIAL:
|
20 ml
|
| MEDIUM:
|
Not Reported
|
| VOLUME OF MEDIUM:
|
Not Reported
|
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Polytron PT1200 (did not homogenize sea weed - material wrapped around
generator)
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 10mm generator.
|
| APPLICATION AND RESULTS:
|
PRO200 Homogenizer with l0mm generator worked successfully. It out performed PT1200
|
|
|
| MATERIAL:
|
Catfish
|
| VOLUME OF MATERIAL:
|
Not reported
|
| MEDIUM:
|
None used
|
| VOLUME OF MEDIUM:
|
None used
|
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
| Not reported
|
| EQUIPMENT USED:
|
PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with a 473m1 glass chamber assembly and 2"
diameter blade
|
| APPLICATION AND RESULTS:
|
Successfully homogenized the Catfish.
However, some skin did get wrapped
around the blade.
RECOMMENDATIONS: Equipment supplied was demo equipment and
the
blades may not have been as sharp as a production blade. This could
have contributed to the skin wrapping around the blade.
Recommend using a
sharpened blade. The entire length of the blade should be sharpened, instead
of the standard tips of the blade.
|
|
|
| MATERIAL:
|
Snails
|
| VOLUME OF MATERIAL:
|
5 ml
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Not reported
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 7mm saw tooth generator
|
| APPLICATION AND RESULTS:
|
PRO200 Homogenizer with 7mm saw tooth generator was able to successfully homogenize the mini
snails and their
shells completely.
|
|
|
| MATERIAL:
|
Chicken Gizzard
|
| VOLUME OF MATERIAL:
|
150 grams of tissue
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Brinkmannn Polytron PT10 / 35
|
| EQUIPMENT USED:
|
PRO250 Homogenizer (with stand) or PRO300 D Homogenizer with 30mm generator
|
| APPLICATION AND RESULTS:
|
Successfully homogenized the chicken
gizzards with 30mm generator.
|
|
|
| MATERIAL:
|
Rat Liver
|
| VOLUME OF MATERIAL:
|
150 grams of tissue
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Brinkmannn Polytron PT10 / 35
|
| EQUIPMENT USED:
|
PRO250 Homogenizer with stand or PRO300 D Homogenizer with 30mm generator.
|
| APPLICATION AND RESULTS:
|
Successfully homogenized the rat liver.
|
|
|
| MATERIAL:
|
Bovine brain
|
| VOLUME OF MATERIAL:
|
50 g (10mm gen.) and 150 g (37mm gen.)
|
| MEDIUM:
|
Not reported
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Homogenization
|
| PREVIOUS OR CURRENT METHOD:
|
Brinkmannn Polytron PT10 / 35
|
| EQUIPMENT USED:
|
PRO250 Homogenizer with stand or PRO300 D Homogenizer with l0mm and 30mm
Generator
|
| APPLICATION AND RESULTS:
|
Successfully homogenized the Bovine brain
with both volumes and
generators.
|
|
|
| MATERIAL:
|
Popcorn, Hot air popped
|
| VOLUME OF MATERIAL:
|
400 ml
|
| MEDIUM:
|
None
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
Process this material to a level of consistent particle size - ideally this
material should pass thru a 40 mesh.
|
| PREVIOUS OR CURRENT METHOD:
|
Unknown
|
| EQUIPMENT USED:
|
PRO250 Homogenizer with stand or PR0300 D Homogenizer with a 30mm rotor stator generator. The sample
was placed in a 473ml open glass container.
|
| APPLICATION AND RESULTS:
|
The sample was placed in a 473m1 glass
container, running at 28,000 rpm. The
container was handheld and moved around as well as up and down to allow the
generator to cover the total container. The total running time was 45
seconds. While running the sample the top of the container had to
be covered to keep the light powder material from being thrown out.
|
|
|
| MATERIAL:
|
Popcorn, Hot air popped
|
| VOLUME OF MATERIAL:
|
300 - 400m1
|
| MEDIUM:
|
NA
|
| VOLUME OF MEDIUM:
|
NA
|
| PROCESS:
|
Process this material to a level of consistent particle size - ideally this
material should pass thru a 40 mesh.
|
| PREVIOUS OR CURRENT METHOD:
|
Unknown
|
| EQUIPMENT USED:
|
PRO250 Homogenizer with stand or PR0300 D Homogenizer with a 2" diameter blade within a 473ml-sealed
container.
|
| APPLICATION AND RESULTS:
|
Tests: 1-3 at 300m1 of material. Test: 4
at 400m1 of material.
Test 1:
10,000 rpm - 15/20 seconds, 28,000 rpm - 40/45 seconds on & off.
Test
2: Varied speeds for 5 minutes - on & off.
Test 3: 10,000 rpm - 1 minute,
stopped, 20,000 rpm - 1 minute, stopped, 28,000 rpm - 1 minute.
Test 4:
10,000 rpm - 1 minute, stopped, 20,000 rpm - 1 minute, stopped, 28,000 rpm -
1 minute.
RECOMMENDATIONS: This method seemed to work better than using
an Open container homogenizing system. There was less mess and
the container did not have to be moved around.
|
|
|
| MATERIAL:
|
Peanut butter, defatted and dried
|
| VOLUME OF MATERIAL:
|
40 ml of unpacked material
|
| MEDIUM:
|
None
|
| VOLUME OF MEDIUM:
|
None
|
| PROCESS:
|
Process this material to a level of consistent
particle size-ideally particle
size of this material should pass thru a 40 mesh.
|
| PREVIOUS OR CURRENT METHOD:
|
Unknown
|
| EQUIPMENT USED:
|
PRO250 Homogenizer with stand or PR0300 D Homogenizer with the following:
T1: 20mm and 30mm generator
run in a 150m1 glass beaker.
T2: 30mm generator run in a 150m1 glass
beaker.
T3: 30mm generator run in a 150m1 glass beaker.
|
| APPLICATION AND RESULTS:
|
Various tests were run as follows:
Test 1: Ran at 28,000 rpm for 1 minute –
up and down. Stopped. Ran at 28,000 rpm for 1 minute – up and down.
Test 2:
Ran at 10,000 rpm for 1 minute. Stopped. Ran at 28,000 rpm for 1 minute.
Test
3: Ran at 28,000 rpm for 1-½ minutes.
RECOMMENDATIONS:
Test 1: The 30mm
generator seemed to work as well as the 20mm generator, so we would recommend
using only one generator.
Test 2: This test proved to us that running just
the 30mm generator for 2 minutes, was as good as running both a 20mm as well
as a 30mm generator for 2 minutes.
Test 3: This test was done to determine
the level of homogenization that was done in 1 1/2 minutes compared to
the samples in tests 1 and 2, which were run for 2 minutes.
Same
results.
|
|
|
| MATERIAL:
|
Peanut butter, defatted and dried
|
| VOLUME OF MATERIAL:
|
120 ml of unpacked material.
|
| MEDIUM:
|
None
|
| VOLUME OF MEDIUM:
|
None
|
| PROCESS:
|
Process this material to a level of consistent particle size - ideally this
material should pass thru a 40 mesh.
|
| PREVIOUS OR CURRENT METHOD:
|
Unknown
|
| EQUIPMENT USED:
|
PRO250 (w/ stand) or PRO300 with a 2" diameter blade attached to a 473m1
sealed glass container.
|
| APPLICATION AND RESULTS:
|
Test 1: 120m1, 28,000 rpm for 30 sec.
Stop. Remove and shake the container,
28,000 rpm for 30 sec. Stop.
Test 2: 120m1 at 28,000 rpm for 20 sec. Stop.
Remove and shake the container. Remove all but 40m1 of material. Run at
28,000 rpm for 20 sec.
Test 3: Balance of 120m1 from test 2 at 28,000 rpm
for 20 seconds. Then in a 50 m1 conical tube at 28,000 rpm for 45
seconds.
RECOMMENDATIONS:
1: This method of using a sealed container with
a blade was better than an Open container homogenizing system. This test was
run to see how small of a sample can be homogenized using a blade.
This test was run to see how the sample homogenized using a blade.
This test was run to see how homogenized using a blade without doing
any
shaking.
|
|
|
| MATERIAL:
|
Rice seeds (grain)
|
| VOLUME OF MATERIAL:
|
5:1 ratio to water
|
| MEDIUM:
|
Water
|
| VOLUME OF MEDIUM:
|
Not reported
|
| PROCESS:
|
To get out the protein
|
| PREVIOUS OR CURRENT METHOD:
|
Unknown
|
| EQUIPMENT USED:
|
PRO250 (w/ stand) or PR0300D with 20mm generator
|
| APPLICATION AND RESULTS:
|
Experienced excessive denaturing from air
bubbles.
RECOMMENDATIONS: Air
bubbles were caused by not having the generator deep enough within the
medium. It is recommended that either a smaller generator be used or the
volume of medium be increased.
|
|
|
| MATERIAL:
|
Ticks
|
| VOLUME OF MATERIAL:
|
2 ticks
|
| MEDIUM:
|
PBS/PRS
|
| VOLUME OF MEDIUM:
|
50 ml
|
| PROCESS:
|
Obtain 100% homogenization of the ticks.
|
| PREVIOUS OR CURRENT METHOD:
|
Manual homogenization
|
| EQUIPMENT USED:
|
PRO200 Homogenizer with 5mm generator
|
| APPLICATION AND RESULTS:
|
Homogenized ticks without any problems. System worked very well.
|
|
|
| MATERIAL:
|
Mouse skin
|
| VOLUME OF MATERIAL:
|
200 mg
|
| MEDIUM:
|
Buffered salt solution
|
| VOLUME OF MEDIUM:
|
0.5 m1
|
| PROCESS:
|
Thoroughly homogenize mouse skin to do Beta Galactosidase test.
|
| PREVIOUS OR CURRENT METHOD:
|
Freeze in liquid nitrogen and grind by hand.
|
| EQUIPMENT USED:
|
PRO200 Homogenizer and 7mm generator
|
| APPLICATION AND RESULTS:
|
Results were obtained much faster then by
hand, and the quantitation numbers
compared to the old method of
processing by hand.
|
|
|
| MATERIAL:
|
Aspirin tablets (20 tablets)
|
| VOLUME OF MATERIAL:
|
325mg per tablet
|
| MEDIUM:
|
None
|
| VOLUME OF MEDIUM:
|
None
|
| PROCESS:
|
Homogenize to a fine powder
|
| PREVIOUS OR CURRENT METHOD:
|
Unknown
|
| EQUIPMENT USED:
|
PRO250 Homogenizer with stand or PR0300 D Homogenizer with generators:
Test 1 & 2: 30mm generator
(02-30150)
Test 3 & 4: 20mm generator (02-20150)
|
| APPLICATION AND RESULTS:
|
Various tests were run as follows:
Test 1: 10,000 rpm - 1 minute moving up,
down, and around in a glass jar.
Test 2: 10,000 rpm - 45 seconds moving
up, down, and around in a 150m1 glass beaker.
Test 3: 30,000 rpm - 1
minute moving up, down, and around in a 150ml glass beaker.
Test 4: 30,000
rpm - 35 seconds moving up and down in a 50ml conical
tube.
RECOMMENDATIONS:
Test 1: fine powder with no lumps.
Test 2:
fine powder with no lumps.
Test 3: a powder with some lumps.
Test 4: very
fine powder with no lumps. In all four tests, the aspirinwas reduced to a powder.
|
|
|
|
Contact Us:
99 Willenbrock Rd.
Oxford CT 06478 USA
ph: (203) 267-4600
fax: (203) 267-4606
Copyright © 2004 - 2010 PRO Scientific Inc.
| |