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Homogenizer Applications




Review specific homogenizer applications below or visit any of these general categories:


Biofuel Homogenization

Cardiac Tissue Homogenization

Compounding Homogenizer

Estuarine & Water Research Homogenizers

Food and Agricultural Homogenizers

Frozen Sample Homogenization

PCR Homogenizer

RNA DNA Homogenizer

Tissue Homogenizer



Acetate

Aribidopsis seedlings
PRODUCT APPLICATION REPORT
MATERIAL: 5 ml
VOLUME OF MATERIAL: Aribidopsis seedlings
MEDIUM: Extraction buffer
VOLUME OF MEDIUM: 150 ml
PROCESS: RNA extraction
PREVIOUS OR CURRENT METHOD: Brinkmannn polytron
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Excellent homogenization of entire seedling after grinding with mortar and pestle. Got large yields from the PRO200 Homogenizer  and acceptable RNA samples.


Aspirin tablets (20 tablets)
PRODUCT APPLICATION REPORT
MATERIAL: Aspirin tablets (20 tablets)
VOLUME OF MATERIAL: 325mg per tablet
MEDIUM: None
VOLUME OF MEDIUM: None
PROCESS: Homogenize to a fine powder
PREVIOUS OR CURRENT METHOD: Unknown
EQUIPMENT USED: PRO250 Homogenizer with stand or PR0300 D Homogenizer with generators:
Test 1 & 2: 30mm generator (02-30150)
Test 3 & 4: 20mm generator (02-20150)
APPLICATION AND RESULTS: Various tests were run as follows:

Test 1: 10,000 rpm - 1 minute moving up, down, and around in a glass jar.

Test 2: 10,000 rpm - 45 seconds moving up, down, and around in a 150m1 glass beaker.

Test 3: 30,000 rpm - 1 minute moving up, down, and around in a 150ml glass beaker.
Test 4: 30,000 rpm - 35 seconds moving up and down in a 50ml conical tube.

RECOMMENDATIONS:
Test 1: fine powder with no lumps.
Test 2: fine powder with no lumps.
Test 3: a powder with some lumps.
Test 4: very fine powder with no lumps. In all four tests, the aspirin was reduced to a powder.



Bacteria
PRODUCT APPLICATION REPORT
MATERIAL: Bacteria
VOLUME OF MATERIAL: 500ml
MEDIUM: Culture
VOLUME OF MEDIUM: Not reported
PROCESS: Complete dispersion with low foaming
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron
EQUIPMENT USED: PRO200 Homogenizer with 20mm PRO Safety Sealed Chamber Assembly
APPLICATION AND RESULTS: Worked perfectly with minimal foaming.


Bacterial phase-filaments
PRODUCT APPLICATION REPORT
MATERIAL: Bacterial phage-filaments
VOLUME OF MATERIAL: .015-.02 ml
MEDIUM: Aqueous buffer
VOLUME OF MEDIUM: 1-1.5 ml
PROCESS: disruption of the filament
PREVIOUS OR CURRENT METHOD: Mechanical Shear and Hand-press
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Achieved desired results in 25-30 sec.


Biological tissue
PRODUCT APPLICATION REPORT
MATERIAL: Biological tissue
VOLUME OF MATERIAL: 5ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Break down tissue for analysis and testing
PREVIOUS OR CURRENT METHOD: Hand mixer
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: Achieved desired results


Bone and joint tissue
PRODUCT APPLICATION REPORT
MATERIAL: Bone and joint tissue
VOLUME OF MATERIAL: Micro volumes
MEDIUM: Tween-80 in TSB
VOLUME OF MEDIUM: Micro volumes
PROCESS: Bacteria in the bone and joint tissue were isolated
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer
APPLICATION AND RESULTS: Achieved desired results for overnight culture


Bovine brain
PRODUCT APPLICATION REPORT
MATERIAL: Bovine brain
VOLUME OF MATERIAL: 50 g (10mm gen.) and 150 g (37mm gen.)
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron PT10 / 35
EQUIPMENT USED: PRO250 Homogenizer with stand or PRO300 D Homogenizer with l0mm and 30mm
Generator
APPLICATION AND RESULTS: Successfully homogenized the Bovine brain
with both volumes and generators.


Bovine thyroid tissue
PRODUCT APPLICATION REPORT
MATERIAL: Bovine thyroid tissue
VOLUME OF MATERIAL: 50ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Worked better than expected.


Cat Fish
PRODUCT APPLICATION REPORT
MATERIAL: Catfish
VOLUME OF MATERIAL: Not reported
MEDIUM: None used
VOLUME OF MEDIUM: None used
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with a 473m1 glass chamber assembly and 2" diameter blade
APPLICATION AND RESULTS: Successfully homogenized the Catfish.
However, some skin did get wrapped around the blade.
RECOMMENDATIONS: Equipment supplied was demo equipment and the
blades may not have been as sharp as a production blade. This could have contributed to the skin wrapping around the blade.
Recommend using a sharpened blade. The entire length of the blade should be sharpened, instead of the standard tips of the blade.


Cell membrane
PRODUCT APPLICATION REPORT
MATERIAL: Cell membrane
VOLUME OF MATERIAL: 50 ml
MEDIUM: PBS type
VOLUME OF MEDIUM: 50 ml
PROCESS: Cell membrane preparation
PREVIOUS OR CURRENT METHOD: Large homogenizers
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Quicker and easier than other homogenizers along with achieving same results.


Cells
PRODUCT APPLICATION REPORT
MATERIAL: Cells
VOLUME OF MATERIAL: .5 ml
MEDIUM:  Not reported
VOLUME OF MEDIUM: 5 ml
PROCESS: Break open cell membranes
PREVIOUS OR CURRENT METHOD: Freezing and thawing
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Completely homogenized and broken down in
fifteen seconds.


Chicken cartilage
PRODUCT APPLICATION REPORT
MATERIAL: Chicken cartilage
VOLUME OF MATERIAL: Not reported
MEDIUM: Sucrose
VOLUME OF MEDIUM: 1/2 ml
PROCESS: Homogenization without destroying the sub-cellular
components
PREVIOUS OR CURRENT METHOD: Dounce homogenizer
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Ran well and kept sub-cellular components


Chicken gizzards
PRODUCT APPLICATION REPORT
MATERIAL: Chicken gizzards
VOLUME OF MATERIAL: 50g
MEDIUM: Acetone
VOLUME OF MEDIUM: 200 ml
PROCESS: Homogenization for chemical analysis
PREVIOUS OR CURRENT METHOD: Polytron, Tissuemizer
EQUIPMENT USED: PRO250 Homogenizer w/stand or PRO300 Homogenizer with 20mm Generator or Blade PRO Safety Sealed Chamber Assembly
APPLICATION AND RESULTS: Blade system did not completely
homogenize the sample, but results were adequate enough. However,
the 20mm generator completely homogenized the sample in two
minutes after pre-chopping the gizzard into 1 cm pieces.
RECOMMENDATIONS: Cutting large chunks of tissue into pieces
will allow a greater number of applications with the
generators.


Chicken tissue
PRODUCT APPLICATION REPORT
MATERIAL: Chicken tissue
VOLUME OF MATERIAL: 30 mg of tissue
MEDIUM: RLT buffer
VOLUME OF MEDIUM: 600 ul
PROCESS: 30s homogenization twice
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer with MULTI-GEN 7 generator
APPLICATION AND RESULTS: Successfully homogenized for RNA processing
Link DOI: http://dx.doi.org/10.1016/j.cimid.2012.02.004


Chlorophilia
PRODUCT APPLICATION REPORT
MATERIAL: Chlorophilia
VOLUME OF MATERIAL: 5-10 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Water quality testing
PREVIOUS OR CURRENT METHOD: Ultrasonic
EQUIPMENT USED: PRO200 Homogenizer  with 10mm generator
APPLICATION AND RESULTS: Worked in homogenizing the sample


Citrus bark
PRODUCT APPLICATION REPORT
MATERIAL: Citrus bark
VOLUME OF MATERIAL: 5 ml
MEDIUM: Phosphate buffer
VOLUME OF MEDIUM: Not reported
PROCESS: Extraction
PREVIOUS OR CURRENT METHOD: Liquid nitrogen and break up with hammer
EQUIPMENT USED: PRO200 Homogenizer  with 7mm or 10mm generators
APPLICATION AND RESULTS: Able to achieve desired results with both
generator sizes.


Clumped white cell agluts
PRODUCT APPLICATION REPORT
MATERIAL: Clumped white cell agluts
VOLUME OF MATERIAL: Not reported
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Steinbach homogenizer
EQUIPMENT USED: PRO200 Homogenizer  with 7mm or 10mm generators
APPLICATION AND RESULTS: Worked well with both
generator sizes.


Corpus luteum
PRODUCT APPLICATION REPORT
MATERIAL: Corpus luteum
VOLUME OF MATERIAL: Not reported
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization for RNA extraction and RT
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: Bio-Gen PRO200 Homogenizer
APPLICATION AND RESULTS:
DOI:

The CLs were homogenized with TRIzol Reagent 

http://dx/doi.org/10.1530/REP-13-0398




Cotton roots
PRODUCT APPLICATION REPORT
MATERIAL: Cotton roots
VOLUME OF MATERIAL: 5-6 ml
MEDIUM: SPW
VOLUME OF MEDIUM: 5-6 ml
PROCESS: Reduce in size and release hormones
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer  with 10mm generator
APPLICATION AND RESULTS: Worked well with tender roots, but not as
effective with hardened stems. For best results use a tube a
little larger in diameter than the generator


Cranberries
PRODUCT APPLICATION REPORT
MATERIAL: Cranberries
VOLUME OF MATERIAL: 30 ml
MEDIUM: Water
VOLUME OF MEDIUM: 20 ml
PROCESS: Homogenization of berries in robot assembly line
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with blade and sealed
chamber assembly
APPLICATION AND RESULTS: Worked well


Cultured bone marrow macrophagecells
PRODUCT APPLICATION REPORT
MATERIAL: Cultured bone marrow macrophage cells
VOLUME OF MATERIAL: Not reported
MEDIUM: Phosphate buffered saline
VOLUME OF MEDIUM: 2.25 ml
PROCESS: Membrane suspension from whole cell suspension
PREVIOUS OR CURRENT METHOD: Ultrasound, barrel and pestle
EQUIPMENT USED: PRO200 Homogenizer  with l0mm generator
APPLICATION AND RESULTS: With old system, only able to achieve 400
rpms of binding above the blank value of 100. With the PRO200 Homogenizer
system, achieved 4000 rpms within seconds. However, results were
too good for the experiment that was being conducted.


Drosophilia aelanogaster
PRODUCT APPLICATION REPORT
MATERIAL: Drosophilia melanogaster
VOLUME OF MATERIAL: 3-10 fruit flies
MEDIUM: Aqueous buffer
VOLUME OF MEDIUM: 100-300 ml
PROCESS: Complete homogenization and release of DNA for
polymerize chain-reaction
PREVIOUS OR CURRENT METHOD: Grinding with glass rod or
applicator sticks
EQUIPMENT USED: PRO200 Homogenizer  with 5mm generator
APPLICATION AND RESULTS: Worked well in grinding the fruit flies
and preservation of DNA, however, scientists were concerned over
the mixing of DNA molecules from different strains as material
stuck to the generators during processing.
RECOMMENDATIONS: Generators can be completely immersed and
sterilized between applications with minimal disassembly.


Ecoli cells
PRODUCT APPLICATION REPORT
MATERIAL: E. Coli cells
VOLUME OF MATERIAL: 25 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Breakage of cell (100%)
PREVIOUS OR CURRENT METHOD: Ultrasonic homogenizer
EQUIPMENT USED: PRO200 Homogenizer  with 5mm generator
APPLICATION AND RESULTS: Did not get 100% breakage in 15 seconds.
Sometimes longer running times are needed to
obtain complete homogenization. Fifteen seconds is not quite long
enough for the type of disruption needed for this application.


Escherichia coil
PRODUCT APPLICATION REPORT
MATERIAL: Escherichia coli
VOLUME OF MATERIAL: Not reported
MEDIUM: Chemical solution
VOLUME OF MEDIUM: Not reported
PROCESS: Isolation of fimbriae
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO200 Homogenizer  with 20mm generator
APPLICATION AND RESULTS: The 20mm generator produced the required
homogenization result.


Fall Armyworm
PRODUCT APPLICATION REPORT

MATERIAL: Fall Armyworm
VOLUME OF MATERIAL:
MEDIUM: 4 ml of phosphate buffered saline
VOLUME OF MEDIUM: 15 ml tube
PROCESS: Homogenized
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO Homogenizer 
APPLICATION AND RESULTS:

DOI:
Individual speciments were homogenized in 4 ml of phosphate buffered saline in a 15ml test tube using a tissue homogenizer. 

http://dx.doi.org/10.1653/024.096.0305


Fig Newton cookies
PRODUCT APPLICATION REPORT
MATERIAL: Fig Newton cookies
VOLUME OF MATERIAL: 20 grams
MEDIUM: None
VOLUME OF MEDIUM: Not reported
PROCESS: Grinding to measure moisture
PREVIOUS OR CURRENT METHOD: Waring blender
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300D with sealed chamber
APPLICATION AND RESULTS: Able to grind the Fig Newton, but lack of
medium caused the paste within the cookie to become heated and
reduces the moisture content, which had comparable results to the
blender. A medium is recommended.


Fish oil
PRODUCT APPLICATION REPORT
MATERIAL: Fish oil
VOLUME OF MATERIAL: Not reported
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenize for future application
PREVIOUS OR CURRENT METHOD: Mechanical and ultrasonic homogenizer
EQUIPMENT USED: PRO250 Homogenizer with speed control box
APPLICATION AND RESULTS:



DOI:
The hot emulsion resulted by mixing the lipid
and aqueous phases at 85°C was exposed
to an external mechanical energy by high shear homogenization.

http://www.scientificbulletin.upb.ro/rev_docs_arhiva/rezc5e_246325.pdf





Fish tissue
PRODUCT APPLICATION REPORT
MATERIAL: Fish tissue
VOLUME OF MATERIAL: 10-400 ml
MEDIUM: Water
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenize for future application
PREVIOUS OR CURRENT METHOD: Hand and ultrasonic homogenizer
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with sealed
chamber assembly
APPLICATION AND RESULTS: Worked well and sealed system proved to
be essential success of application.


Food additives
PRODUCT APPLICATION REPORT
MATERIAL: Food additives
VOLUME OF MATERIAL: various
MEDIUM: : 1) Mayonnaise 2) Salad dressing 1 gallon (PRO200 Homogenizer  Process)
VOLUME OF MEDIUM: 1 gallon
PROCESS: Mix food product while adding various additives in liquid
and powder form to obtain vertical flow.
PREVIOUS OR CURRENT METHOD: Waring blender
EQUIPMENT USED: PRO200 Homogenizer  with 20mm generator and PRO300 D Homogenizer with a
blade and sealed chamber.
APPLICATION AND RESULTS: The PRO200 Homogenizer  and the 20mm generator were
unable to mix the mayonnaise and the secret additive, however it
worked superbly on the salad dressing. The PRO300 D Homogenizer and the blade
system were used on the mayonnaise and began to mix but there was
no vertical flow of material.

RECOMMENDATIONS: Recommend that a larger diameter generator and
PRO250 Homogenizer or PRO300 D Homogenizer be used to cause vertical flow of the material.


Food staples
PRODUCT APPLICATION REPORT
MATERIAL: Food samples
VOLUME OF MATERIAL: 1 ml
MEDIUM: Organic aqueous
VOLUME OF MEDIUM: 5 ml
PROCESS: Complete dispersion
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO200 Homogenizer  with 7mm or 10mm generator
APPLICATION AND RESULTS: Good homogenization results


Freshwater snail
PRODUCT APPLICATION REPORT
MATERIAL: Freshwater snail
VOLUME OF MATERIAL: Not reported
MEDIUM: distilled water
VOLUME OF MEDIUM: 0.5 ml
PROCESS: Homogenized
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO200 Homogenizer  
APPLICATION AND RESULTS:

DOI:
Each body was weighed, transferred to a flat bottom tube and homogenized for 15 s. 


http://dx/doi.org/10.1080/00288330.2013.846921




Frog embryos
PRODUCT APPLICATION REPORT
MATERIAL: Frog embryos
VOLUME OF MATERIAL: Not reported
MEDIUM: varied
VOLUME OF MEDIUM: 1.5 ml
PROCESS: Cell disruption
PREVIOUS OR CURRENT METHOD: Mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer  with 7mm or 10mm generators
APPLICATION AND RESULTS: Worked well.


Frozen rat heart
PRODUCT APPLICATION REPORT
MATERIAL: Frozen rat heart
VOLUME OF MATERIAL: 130-150 mg
MEDIUM: HEPES, EDTA, DTT, MgCl2
VOLUME OF MEDIUM: 25-30mM
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer
APPLICATION AND RESULTS: Achieved desired results
Link DOI: http://dx.doi.org/10.1002/jcp.24079


Fungi
PRODUCT APPLICATION REPORT
MATERIAL: 50 ml
VOLUME OF MATERIAL: Not reported
MEDIUM: Not reported
VOLUME OF MEDIUM: 50 ml
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer  with 10mm generator
APPLICATION AND RESULTS: Worked well.


Gypsy Moth eggs
PRODUCT APPLICATION REPORT
MATERIAL: Gypsy Moth eggs
VOLUME OF MATERIAL: 05 ml
MEDIUM: Aqueous
VOLUME OF MEDIUM: 1 ml
PROCESS: Rupture of eggs
PREVIOUS OR CURRENT METHOD: Manual
EQUIPMENT USED: PRO200 Homogenizer  with 5mm generator
APPLICATION AND RESULTS: Successfully ruptured eggs for further
analysis.


Hair shampoo
PRODUCT APPLICATION REPORT
MATERIAL: Hair shampoo
VOLUME OF MATERIAL: 15000-20000g
MEDIUM: None
VOLUME OF MEDIUM: None
PROCESS: Mix shampoo to desired consistency
PREVIOUS OR CURRENT METHOD: Large mixer with 24" rotor and
4" blade
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with a blade and
sealed chamber assembly
APPLICATION AND RESULTS: The blade moved a great deal of the
shampoo because the high rpms.


Hamster brain
PRODUCT APPLICATION REPORT
MATERIAL: Hamster brain
VOLUME OF MATERIAL: 10 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization for RNA and protein purification
analysis
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer  with 10mm generator
APPLICATION AND RESULTS: Successful completion of application.


Hamster tissue
PRODUCT APPLICATION REPORT
MATERIAL: Hamster tissue
VOLUME OF MATERIAL: 9 grams
MEDIUM: Aqueous
VOLUME OF MEDIUM: 1-5 ml
PROCESS: Release bacteria from tissue
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO200 Homogenizer  with a 10mm generator
APPLICATION AND RESULTS: Required results achieved easily.


Heart cells
PRODUCT APPLICATION REPORT
MATERIAL: Heart cells
VOLUME OF MATERIAL: Not reported
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Isolation of metabolites
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO200 Homogenizer  with 5mm generator
APPLICATION AND RESULTS: Worked well. Intended results achieved
within seconds.


Hippocampus and prefrontal cortex 
PRODUCT APPLICATION REPORT
MATERIAL: Hippocampus and prefrontal cortex 
VOLUME OF MATERIAL: 1 ml
MEDIUM:
VOLUME OF MEDIUM: Not reported
PROCESS:
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO Homogenizer
APPLICATION AND RESULTS:

DOI:
Hippocampus and prefrontol cortex and
1.0ml for nucleus accumbens, using a handheld homogenizer on slow setting.  

http://dx.doi.org/10.1007/s00216-013-7514-9




HIV Infected H9 cells
PRODUCT APPLICATION REPORT
MATERIAL: HIV Infected H9 cells
VOLUME OF MATERIAL: 20-200 g
MEDIUM: Not reported
VOLUME OF MEDIUM: 50-400 ml
PROCESS: Disrupt cell membrane
PREVIOUS OR CURRENT METHOD: Dounce Homogenizer
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with blade and sealed
chamber assembly
APPLICATION AND RESULTS: Worked well and sealed system is a
must. However, sharpened blades may pose a huge health risk if
poked into skin.

RECOMMENDATIONS: As an added safety feature for working with a
such material, we would suggest the use of a generator system
(smooth bottomed) that has no exposed sharp edges that can break
the skin of its user.


Human liver
PRODUCT APPLICATION REPORT
MATERIAL: Human liver
VOLUME OF MATERIAL: 100-200 ml
MEDIUM: Water
VOLUME OF MEDIUM: Not reported
PROCESS: Complete homogenization
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with 1) blade and
sealed chamber assembly 2) 20mm generator
APPLICATION AND RESULTS: Worked best with the blade and sealed
chamber assembly. Chunks were too big for the 20mm generator.


Human tissue
PRODUCT APPLICATION REPORT
MATERIAL: Human tissue
VOLUME OF MATERIAL: 5 g
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Brinkmannn homogenizer
EQUIPMENT USED: PRO200 Homogenizer  with 10mm generator
APPLICATION AND RESULTS: Homogenized tissue well and was quieter
than previous methods.


Human tissue
PRODUCT APPLICATION REPORT
MATERIAL: Human tissue
VOLUME OF MATERIAL: .05 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenize RNA
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer  with 5mm generator
APPLICATION AND RESULTS: Worked well


Human tissue
PRODUCT APPLICATION REPORT
MATERIAL: Human tissue
VOLUME OF MATERIAL: 8 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: RNA extraction
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron
EQUIPMENT USED: PRO200 Homogenizer  with 7mm generators
APPLICATION AND RESULTS: Liked low noise level of units. Achieved
desired results.


Human tissue
PRODUCT APPLICATION REPORT
MATERIAL: Human tissue
VOLUME OF MATERIAL: 20 ml
MEDIUM: Phosphate buffer
VOLUME OF MEDIUM: 50 ml
PROCESS: Complete homogenization
PREVIOUS OR CURRENT METHOD: shift blender, mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer  with 10mm generators
APPLICATION AND RESULTS: Worked very well and desired results were
achieved.


Intestinal Samples (Swine)
PRODUCT APPLICATION REPORT


MATERIAL: Intestinal samples (Swine)
VOLUME OF MATERIAL: 2mm pieces
MEDIUM:
VOLUME OF MEDIUM:
PROCESS: Lysed
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO200 Homogenizer
APPLICATION AND RESULTS:


DOI:
To obtain representative measurements from the
2-cm jejunal samples, three cross-sections of
approximately 
2 mm were pooled and lysed using
PRO 200 Post-Mounted Laboratory

http://dx.doi.org/10.1371/journal.pone.0079343


Kidneys
PRODUCT APPLICATION REPORT


MATERIAL: Kidneys
VOLUME OF MATERIAL:
MEDIUM: PBS
VOLUME OF MEDIUM: 5 ml
PROCESS: Complete homogenization
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO Homogenizer
APPLICATION AND RESULTS:


DOI:
Kidneys excised for fungal burden assessments 
were weighed, placed in 5 ml PBS, and homogenized
for 30 s with a tissue homogenizer. 

http://dx.doi.org/10.1128/mBio.00723-13


Kidneys
PRODUCT APPLICATION REPORT


MATERIAL: Kidneys
VOLUME OF MATERIAL:
MEDIUM:
VOLUME OF MEDIUM:
PROCESS: For Western blotting, grinded, quantified and then homogenized. 
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO Homogenizer
APPLICATION AND RESULTS:


DOI:
To investigate HSP expression, some portion of the isolated right and left kidneys were cut into
pieces and stored at −72°C. For Western blotting, the stored kidney pieces were grinded, quantified, and then homogenized. 

http://dx/doi.org/10.1016/j.transproceed.2013.08.028




Larval samples
PRODUCT APPLICATION REPORT
MATERIAL: Larval samples
VOLUME OF MATERIAL:
MEDIUM: cold Tri- HCl buffer
VOLUME OF MEDIUM: 4:1 v/w 
PROCESS: Homogenized
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO200 Homogenizer  
APPLICATION AND RESULTS:

DOI:
Larval samples were homogenized using a tissue homogenizer.

http://dx/doi.org/10.1007/s10695-013-9884-5




Leaves
PRODUCT APPLICATION REPORT
MATERIAL: Leaves
VOLUME OF MATERIAL: 25 ml
MEDIUM: Organic buffer
VOLUME OF MEDIUM: 75 ml
PROCESS: Grinding of leaves for protein analysis
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron
EQUIPMENT USED: PRO200 Homogenizer  with 10mm generator
APPLICATION AND RESULTS: Good results.


Lipid and aqueous phases
PRODUCT APPLICATION REPORT
MATERIAL: Lipid and aqueous phases
VOLUME OF MATERIAL: Not reported
MEDIUM: Lipid and aqueous phases
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenized for 5 minutes
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO250 Homogenizer
APPLICATION AND RESULTS: Blended by a high-shear homogenizer


Lipid and aqueous phases
PRODUCT APPLICATION REPORT
MATERIAL: Lipid and aqueous phases
VOLUME OF MATERIAL: Not reported
MEDIUM: Lipid and aqueous phases
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenized for 5 minutes
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO250 Homogenizer
APPLICATION AND RESULTS:

DOI:
Aqueuous phase was added to lipid phase and mixed under homogenization at 12,000 rpm with PRO250.  

http://dx.doi.org/10.1208/s12248-013-9550-y




Liver cells from rat
PRODUCT APPLICATION REPORT
MATERIAL: Liver cells from rat
VOLUME OF MATERIAL: 10 ml
MEDIUM: Krebs-Ringer buffer
VOLUME OF MEDIUM: 20 ml
PROCESS: Rupturing of hepatocytes and leaving of sub-cellular
components intact
PREVIOUS OR CURRENT METHOD: Dounce homogenizer
EQUIPMENT USED: PRO200 Homogenizer  with 1Omm generator
APPLICATION AND RESULTS: The PRO200 Homogenizer  ruptured the cells in a very
short time (2 minutes) leaving the sub-cellular components
intact. There was minimal foaming and the compactness and noise
level of the instrument were added benefits


Liver tissue (mouse)
PRODUCT APPLICATION REPORT
MATERIAL: Liver tissue (mouse)
VOLUME OF MATERIAL: .5-5 ml
MEDIUM: PBS
VOLUME OF MEDIUM: Not reported
PROCESS:
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO Homogenizer
APPLICATION AND RESULTS:

DOI:
Liver tissue was homogenized on ice for 30 s. 

http://dx/doi.org/10.1021/jf403173k




Lymphocyte brain and thymus
PRODUCT APPLICATION REPORT
MATERIAL: Lymphocyte, brain, and thymus
VOLUME OF MATERIAL: .5-5 ml
MEDIUM: GITC buffer
VOLUME OF MEDIUM: Not reported
PROCESS: Isolation of RNA and DNA
PREVIOUS OR CURRENT METHOD: Mortar and pestle, blender
EQUIPMENT USED: PRO200 with 5mm or 7mn generators
APPLICATION AND RESULTS: Application successful
with either generator between the volumes of
material.


Mice lungs
PRODUCT APPLICATION REPORT
MATERIAL: Mice lungs
MEDIUM: 50 ml
VOLUME OF MEDIUM: 100 ml
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Virtis
EQUIPMENT USED: PRO200 with 10mm generator
APPLICATION AND RESULTS: Heating was a concern, but none resulted
and the experiment was completed successfully.


Mango seed kernel

PRODUCT APPLICATION REPORT
MATERIAL: Mango seed kernel
VOLUME OF MATERIAL: Not reported
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Effects of encapsulation materials, including polysaccharides and proteins
PREVIOUS OR CURRENT METHOD: Not Reported
EQUIPMENT USED: PRO200
APPLICATION AND RESULTS: 10,000rpm for 2 minutes
Link DOI: http://dx.doi.org/10.1016/j.foodchem.2012.03.054


Mosquitoes

PRODUCT APPLICATION REPORT
MATERIAL: Mosquitoes
VOLUME OF MATERIAL: 50 mosquitoes
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Virus analysis in mosquitoes
PREVIOUS OR CURRENT METHOD: Mortar and pestle
EQUIPMENT USED: PRO200 with 5mm generator
APPLICATION AND RESULTS: PRO200 saves considerable amount of time
and works well in this application.


Mouse ears
PRODUCT APPLICATION REPORT
MATERIAL: Mouse ears
VOLUME OF MATERIAL: 1-5 ml
MEDIUM: Buffer solution
VOLUME OF MEDIUM: Not reported
PROCESS: Cell disruption
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron
EQUIPMENT USED: PRO200 with 5mm or 7mm generators
APPLICATION AND RESULTS: Cell disruption and adequate sample size
achieved with both generator sizes (generator diameter depended on
material volume), which had considerably less noise than Polytron. 


Mouse liver, spleen, lung and skin
PRODUCT APPLICATION REPORT
MATERIAL: Mouse liver, spleen, lung and skin
VOLUME OF MATERIAL: 1 cm2
MEDIUM: sterile PBS
VOLUME OF MEDIUM: 2 ml
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer
APPLICATION AND RESULTS: Achieved desired homogenization results for overnight incubation/ colonization


Mouse muscle
PRODUCT APPLICATION REPORT
MATERIAL: Mouse muscle
VOLUME OF MATERIAL: 2 ml
MEDIUM: Body fluids
VOLUME OF MEDIUM: 4 ml
PROCESS: Not reported
PREVIOUS OR CURRENT METHOD: Mortar and Pestle
EQUIPMENT USED: PRO200 Homogenizer  with 7mm generator
APPLICATION AND RESULTS: Results were acceptable


Mouse organs
PRODUCT APPLICATION REPORT
MATERIAL: Mouse organs
VOLUME OF MATERIAL: 10 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Stirring motor with grinder
EQUIPMENT USED: PRO200 Homogenizer  with a 10mm generator
APPLICATION AND RESULTS: The PRO200 Homogenizer  successfully completed the
application.


Mouse skin
PRODUCT APPLICATION REPORT
MATERIAL: Mouse skin
VOLUME OF MATERIAL: 200 mg
MEDIUM: Buffered salt solution
VOLUME OF MEDIUM: 0.5 m1
PROCESS: Thoroughly homogenize mouse skin to do Beta Galactosidase test.
PREVIOUS OR CURRENT METHOD: Freeze in liquid nitrogen and grind by hand.
EQUIPMENT USED: PRO200 Homogenizer and 7mm generator
APPLICATION AND RESULTS: Results were obtained much faster then by
hand, and the quantitation numbers compared to the old method of
processing by hand.


Muscle brain connective tissue
PRODUCT APPLICATION REPORT
MATERIAL: Muscle brain connective tissue
VOLUME OF MATERIAL: 50ml
MEDIUM: Water
VOLUME OF MEDIUM: 100 ml
PROCESS: Pulverize or powder frozen muscle tissue
PREVIOUS OR CURRENT METHOD: Tekmar Tissu-mizer
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300D a 20mm generator
APPLICATION AND RESULTS: Generator was able to provide successful
results.


Muscle tissue
PRODUCT APPLICATION REPORT
MATERIAL: Muscle tissue
VOLUME OF MATERIAL: <.5 ml
MEDIUM: Aqueous buffer
VOLUME OF MEDIUM: 2 ml
PROCESS: Extraction
PREVIOUS OR CURRENT METHOD: Mortar and pestle after freezing
sample with liquid nitrogen
EQUIPMENT USED: PRO200 with 5mm or 7mm generators
APPLICATION AND RESULTS: Both generators work within an
Eppendorf tube. Both generators produce the same good
results, because of small volume of material. Emulsion will
exists in larger samples.


Mustard and pepper buds
PRODUCT APPLICATION REPORT
MATERIAL: Mustard and pepper buds
VOLUME OF MATERIAL: 20 total buds
MEDIUM: Buffer
VOLUME OF MEDIUM: 30 ml
PROCESS: Release of microspores and removal of supernatant
PREVIOUS OR CURRENT METHOD: Mortar and pestle
EQUIPMENT USED: PRO200 with l0nm generator
APPLICATION AND RESULTS: Dispersion was more than adequate.


Nerve segments
PRODUCT APPLICATION REPORT
MATERIAL: Nerve segments
VOLUME OF MATERIAL: .1 ml (5mm gen.) and 75 ml (10mm gen.)
MEDIUM: SDS buffer
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Manual grinding and glass-glass
microhomogenizer
EQUIPMENT USED: PRO200 Homogenizer  with 5mm and 10mm generators
APPLICATION AND RESULTS: Application successful.


Nerve tissue
PRODUCT APPLICATION REPORT
MATERIAL: Nerve tissue
VOLUME OF MATERIAL: .1 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Wheaton mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer  with 5mm generator
APPLICATION AND RESULTS: Tissue was too stringy and offered no
resistance to the blade.
RECOMMENDATIONS: In situations where the material is too flexible
or stringy, liquid nitrogen can be used to freeze the sample. The
blade will then be able to cut through the frozen sample. The
liquid nitrogen allows for the sample to be grinded very finely. A
7mm saw tooth bottom generator would also be recommended, when
liquid nitrogen is not available.


Nuts
PRODUCT APPLICATION REPORT
MATERIAL: Nuts
VOLUME OF MATERIAL: 1 g
MEDIUM: Water
VOLUME OF MEDIUM: 20 ml
PROCESS: Homogenization to recover enzymes
PREVIOUS OR CURRENT METHOD: Mortar and pestle and then vortex
stirrer
EQUIPMENT USED: PRO200 Homogenizer  with 10mm generator
APPLICATION AND RESULTS: Nuts were ground successfully.


Olfactory bulb
PRODUCT APPLICATION REPORT
MATERIAL: Olfactory bulb
VOLUME OF MATERIAL:
MEDIUM: Protease inhibitors were add to a homogenization buffer
VOLUME OF MEDIUM:
PROCESS:
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO Homogenizer  
APPLICATION AND RESULTS:

DOI:
Samples were homogenized by 50 strokes on ice.

http://dx.doi.org/10.1016/j.neuroscience.2013.12.033




Ovarian tissue
PRODUCT APPLICATION REPORT
MATERIAL: Ovarian tissue
VOLUME OF MATERIAL: 10 ml
MEDIUM: Phosphate buffer
VOLUME OF MEDIUM: Not reported
PROCESS: RNA and mitochondria isolation
PREVIOUS OR CURRENT METHOD: bounce homogenizer, Brinkmannn
Polytron
EQUIPMENT USED: PRO200 Homogenizer  with 7mm generator
APPLICATION AND RESULTS: Very good for homogenizing small amounts
of tissue. Broke up 75% of cells in defined protocol.


Pancreas virus tissue
PRODUCT APPLICATION REPORT

MATERIAL: Pancreas virus tissue
VOLUME OF MATERIAL: 1 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Extraction
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 with 5mm
APPLICATION AND RESULTS: The PRO200 worked excellent


Parasites
PRODUCT APPLICATION REPORT

MATERIAL: Proteins extracted from parasites
VOLUME OF MATERIAL:
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Extraction
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200
APPLICATION AND RESULTS: Proteins were extracted from the parasites by immersing the parasites in an ice bath, followed by homogenization with a hand-held PRO200


Peanuts and Mumg beans
PRODUCT APPLICATION REPORT
MATERIAL: Peanuts and Mung beans
VOLUME OF MATERIAL: 50 ml
MEDIUM: Aqueous
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization of hypocotyls and seeds to isolate protein
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron
EQUIPMENT USED: PRO200 with 10mm generator
APPLICATION AND RESULTS: Dispersal achieved successfully for the
protein analysis.


Peanut butter defatted and dried
PRODUCT APPLICATION REPORT
MATERIAL: Peanut butter, defatted and dried
VOLUME OF MATERIAL: 40 ml of unpacked material
MEDIUM: None
VOLUME OF MEDIUM: None
PROCESS: Process this material to a level of consistent
particle size-ideally particle size of this material should pass thru a 40 mesh.
PREVIOUS OR CURRENT METHOD: Unknown
EQUIPMENT USED: PRO250 Homogenizer with stand or PR0300 D Homogenizer with the following:

T1: 20mm and 30mm generator run in a 150m1 glass beaker.

T2: 30mm generator run in a 150m1 glass beaker.

T3: 30mm generator run in a 150m1 glass beaker.

APPLICATION AND RESULTS: Various tests were run as follows:

Test 1: Ran at 28,000 rpm for 1 minute – up and down. Stopped. Ran at 28,000 rpm for 1 minute – up and down.

Test 2: Ran at 10,000 rpm for 1 minute. Stopped. Ran at 28,000 rpm for 1 minute.

Test 3: Ran at 28,000 rpm for 1-½ minutes.
RECOMMENDATIONS:

Test 1: The 30mm generator seemed to work as well as the 20mm generator, so we would recommend using only one generator.

Test 2: This test proved to us that running just the 30mm generator for 2 minutes, was as good as running both a 20mm as well as a 30mm generator for 2 minutes.

Test 3: This test was done to determine the level of homogenization that was done in 1 1/2 minutes compared to the samples in tests 1 and 2, which were run for 2 minutes. Same
results.


Peanut butter defatted and dried
PRODUCT APPLICATION REPORT
MATERIAL: Peanut butter, defatted and dried
VOLUME OF MATERIAL: 120 ml of unpacked material.
MEDIUM: None
VOLUME OF MEDIUM: None
PROCESS: Process this material to a level of consistent particle size - ideally this material should pass thru a 40 mesh.
PREVIOUS OR CURRENT METHOD: Unknown
EQUIPMENT USED: PRO250 (w/ stand) or PRO300 with a 2" diameter blade attached to a 473m1 sealed glass container.
APPLICATION AND RESULTS: Test 1: 120m1, 28,000 rpm for 30 sec.
Stop. Remove and shake the container, 28,000 rpm for 30 sec. Stop.

Test 2: 120m1 at 28,000 rpm for 20 sec. Stop. Remove and shake the container. Remove all but 40m1 of material. Run at 28,000 rpm for 20 sec.

Test 3: Balance of 120m1 from test 2 at 28,000 rpm for 20 seconds. Then in a 50 m1 conical tube at 28,000 rpm for 45 seconds.

RECOMMENDATIONS:
1: This method of using a sealed container with a blade was better than an Open container homogenizing system. This test was run to see how small of a sample can be homogenized using a blade. This test was run to see how the sample homogenized using a blade. This test was run to see how homogenized using a blade without doing
any shaking.


Phycomyces
PRODUCT APPLICATION REPORT
MATERIAL: Phycomyces
VOLUME OF MATERIAL: 1 g
MEDIUM: Chloroform, methanol and hydrochloric acid
VOLUME OF MEDIUM: 10 ml
PROCESS: Homogenization of fibers and shear cells
PREVIOUS OR CURRENT METHOD: Mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer  with 7mm generator
APPLICATION AND RESULTS: Worked well, but clogged occasionally.
However, this is not a major problem.


Placenta tissue
PRODUCT APPLICATION REPORT
MATERIAL: Placenta tissue
VOLUME OF MATERIAL: 100 g
MEDIUM: Not reported
VOLUME OF MEDIUM: 2 L
PROCESS: Complete homogenization
PREVIOUS OR CURRENT METHOD: Tekmar Tissue-Mizer
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with 20mm generator
APPLICATION AND RESULTS: Complete homogenization in 15 seconds.


Plant tissue
PRODUCT APPLICATION REPORT
MATERIAL: Plant tissue
VOLUME OF MATERIAL: 50 g
MEDIUM: GTC
VOLUME OF MEDIUM: 100 ml
PROCESS: Homogenization and RNA extraction
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron
EQUIPMENT USED: PRO200 Homogenizer with 20mm generators
APPLICATION AND RESULTS: Worked nicely.


Plant tissue
PRODUCT APPLICATION REPORT
MATERIAL: Plant tissue
VOLUME OF MATERIAL: 10 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Freeze with liquid nitrogen and break
up with more and pestle
EQUIPMENT USED: PRO200 Homogenizer with 7mm generators
APPLICATION AND RESULTS: Successful homogenization


Popcorn Hot air popped
PRODUCT APPLICATION REPORT
MATERIAL: Popcorn, Hot air popped
VOLUME OF MATERIAL: 400 ml
MEDIUM: None
VOLUME OF MEDIUM: Not reported
PROCESS: Process this material to a level of consistent particle size - ideally this material should pass thru a 40 mesh.
PREVIOUS OR CURRENT METHOD: Unknown
EQUIPMENT USED: PRO250 Homogenizer with stand or PR0300 D Homogenizer with a 30mm rotor stator generator. The sample was placed in a 473ml open glass container.
APPLICATION AND RESULTS: The sample was placed in a 473m1 glass
container, running at 28,000 rpm. The container was handheld and moved around as well as up and down to allow the generator to cover the total container. The total running time was 45 seconds. While running the sample the top of the container had to be covered to keep the light powder material from being thrown out. 


Popcorn Hot air popped
PRODUCT APPLICATION REPORT
MATERIAL: Popcorn, Hot air popped
VOLUME OF MATERIAL: 300 - 400m1
MEDIUM: NA
VOLUME OF MEDIUM: NA
PROCESS: Process this material to a level of consistent particle size - ideally this material should pass thru a 40 mesh.
PREVIOUS OR CURRENT METHOD: Unknown
EQUIPMENT USED: PRO250 Homogenizer with stand or PR0300 D Homogenizer with a 2" diameter blade within a 473ml-sealed container.
APPLICATION AND RESULTS: Tests: 1-3 at 300m1 of material. Test: 4
at 400m1 of material.

Test 1: 10,000 rpm - 15/20 seconds, 28,000 rpm - 40/45 seconds on & off.

Test 2: Varied speeds for 5 minutes - on & off.

Test 3: 10,000 rpm - 1 minute, stopped, 20,000 rpm - 1 minute, stopped, 28,000 rpm - 1 minute.

Test 4: 10,000 rpm - 1 minute, stopped, 20,000 rpm - 1 minute, stopped, 28,000 rpm - 1 minute.

RECOMMENDATIONS: This method seemed to work better than using an Open container homogenizing system. There was less mess and the container did not have to be moved around.


Powder
PRODUCT APPLICATION REPORT
MATERIAL: Powder
VOLUME OF MATERIAL: 1 ml
MEDIUM: Aqueous
VOLUME OF MEDIUM: Not Reported
PROCESS: Homogenization of reconstituted polymers
PREVIOUS OR CURRENT METHOD: Hand pestle
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: PRO200 Homogenizer did the job successfully.


Powdered aluminum
PRODUCT APPLICATION REPORT
MATERIAL: Powdered aluminum
VOLUME OF MATERIAL: .5 ml
MEDIUM: None
VOLUME OF MEDIUM: None
PROCESS: Dispersal but no disruption of particle shape or size
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Good application.


Protein lysates
PRODUCT APPLICATION REPORT
MATERIAL: Protein lysates
VOLUME OF MATERIAL:
MEDIUM: Homogenizing solution containing 10 mM Tris pH 8.0,
150 mM NaCl, 
1 mM EDTA, 1 mM orthovanadate,
1% Triton X-100,  
and 60 mM N-octyl-b-D-glucopyranoside,
in the presence of protease inhibitors
VOLUME OF MEDIUM:
PROCESS: lysing
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO200 Homogenizer 
APPLICATION AND RESULTS:








At the end of treatment tumors were fixed in formalin

and embedded in paraffin for IHC or frozen at −80°C for protein lysates. Protein lysates were obtained homogenizing three times

at high speed at 4°C for 20 minutes. 




Quail liver
PRODUCT APPLICATION REPORT
MATERIAL: Quail liver
VOLUME OF MATERIAL: .05 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Grinding
PREVIOUS OR CURRENT METHOD: Freeze with liquid nitrogen and grind
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Worked well.


Rat aorta
PRODUCT APPLICATION REPORT
MATERIAL: Rat aorta
VOLUME OF MATERIAL: Not reported
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Break down rat aorta to recover as much RNA as possible
PREVIOUS OR CURRENT METHOD: Brinkmannn polytron after frozen, and
broken with liquid nitrogen.
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with 10mm generator
APPLICATION AND RESULTS: PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer worked successfully and results were better than previous method.


Rat bowel
PRODUCT APPLICATION REPORT
MATERIAL: Rat bowel
VOLUME OF MATERIAL: 10 g
MEDIUM: TCA
VOLUME OF MEDIUM: Not Reported
PROCESS: Homogenous suspension needed for membrane assays
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron
EQUIPMENT USED: PRO200 Homogenizer  with 10mm generator
APPLICATION AND RESULTS: Worked well and its ease of use were
beneficial in conducting the experiment.


Rat brain
PRODUCT APPLICATION REPORT
MATERIAL: Rat brain
VOLUME OF MATERIAL: 25ml
MEDIUM: PDY buffer
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: PTFE-glass homogenizer
EQUIPMENT USED: PRO200 Homogenizer with an l0mm generator
APPLICATION AND RESULTS: Worked well and cut time drastically.



Rat brain liver muscle peripheral nerves
PRODUCT APPLICATION REPORT
MATERIAL: Rat brain, liver, muscle, peripheral nerves
VOLUME OF MATERIAL: 50 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Protein isolation
PREVIOUS OR CURRENT METHOD: Dounce homogenizer
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Worked very well.


Rat brains
PRODUCT APPLICATION REPORT
MATERIAL: Rat brains
VOLUME OF MATERIAL: 10 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: DNA extraction
PREVIOUS OR CURRENT METHOD: Hand mixer
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: PRO200 Homogenizer achieved desired results and even crushed up the skull that was included in the sample.


Rat epidymous
PRODUCT APPLICATION REPORT
MATERIAL: Rat epidymous
VOLUME OF MATERIAL: 3 ml
MEDIUM: SPW
VOLUME OF MEDIUM: Not reported
PROCESS: Disruption of tissue and release of sperm
PREVIOUS OR CURRENT METHOD: Forceps and scapel
EQUIPMENT USED: PRO200 Homogenizer with 7mm and 10mm generators
APPLICATION AND RESULTS: 7mm too small but best results were
attained with 10mm generator. Total disruption was achieved.


Rat jaw and teeth
PRODUCT APPLICATION REPORT
MATERIAL: Rat jaw and teeth
VOLUME OF MATERIAL: .05 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Completely homogenized sample.


Rat leg muscles
PRODUCT APPLICATION REPORT
MATERIAL: Rat leg muscles
VOLUME OF MATERIAL: 5 ml (7mm gen.) and 20 ml (10mm gen.)
MEDIUM: BSA
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer with 7mm and 10mm generators
APPLICATION AND RESULTS: Ran very well. Saw teeth on 7mm and 10mm
were perfect in homogenizing the sample.


Rat Liver
PRODUCT APPLICATION REPORT
MATERIAL: Rat Liver
VOLUME OF MATERIAL: 150 grams of tissue
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron PT10 / 35
EQUIPMENT USED: PRO250 Homogenizer with stand or PRO300 D Homogenizer with 30mm generator.
APPLICATION AND RESULTS: Successfully homogenized the rat liver. 


Rat spinal cord and root ganglia
PRODUCT APPLICATION REPORT
MATERIAL: Rat spinal cord and root ganglia
VOLUME OF MATERIAL: .5 ml
MEDIUM: Lithium chloride
VOLUME OF MEDIUM: 1 ml
PROCESS: Complete homogenization
PREVIOUS OR CURRENT METHOD: Mortar and Pestle
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: PRO200 worked well on the sample.


Rat tongue and tails
PRODUCT APPLICATION REPORT
MATERIAL: Rat tongue and tails
VOLUME OF MATERIAL: 1 ml
MEDIUM: ECTA, DDT
VOLUME OF MEDIUM: 1.5 ml
PROCESS: Isolate DNA protein
PREVIOUS OR CURRENT METHOD: Grind in blender and Mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer  with 5mm generator
APPLICATION AND RESULTS: Successfully isolated the DNA protein
from the tongue and tails.


Rat uterii
PRODUCT APPLICATION REPORT
MATERIAL: Rat uteri
VOLUME OF MATERIAL: 50 ml
MEDIUM: Water
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Mortar and pestle, Brinkmannn Polytron
EQUIPMENT USED: PRO200 Homogenizer  with 10mm generator
APPLICATION AND RESULTS: Did a better job than the Polytron, and
it produced less noise.


Raw sewage
PRODUCT APPLICATION REPORT
MATERIAL: Raw sewage
VOLUME OF MATERIAL: 5ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Analysis of raw sewage
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: Works well


Retina
PRODUCT APPLICATION REPORT
MATERIAL: Retina
VOLUME OF MATERIAL: Retina was cut into pieces
MEDIUM: Homogenization buffer
VOLUME OF MEDIUM: 200 ul
PROCESS: Not reported
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Successfully homogenized


Rice and barley seeds
PRODUCT APPLICATION REPORT
MATERIAL: Rice and barley seeds
VOLUME OF MATERIAL: 10 ml (both 7mm and 10mm gen. Test)
MEDIUM: Organic solvents
VOLUME OF MEDIUM: Not Reported
PROCESS: Complete dispersal of starch material
PREVIOUS OR CURRENT METHOD: Liquid nitrogen then mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer  with 7mm and 10mm gener
APPLICATION AND RESULTS: Best results with 10mm saw tooth.


Rice seeds (grain)
PRODUCT APPLICATION REPORT
MATERIAL: Rice seeds (grain)
VOLUME OF MATERIAL: 5:1 ratio to water
MEDIUM: Water
VOLUME OF MEDIUM: Not reported
PROCESS: To get out the protein
PREVIOUS OR CURRENT METHOD: Unknown
EQUIPMENT USED: PRO250 (w/ stand) or PR0300D with 20mm generator
APPLICATION AND RESULTS: Experienced excessive denaturing from air
bubbles.
RECOMMENDATIONS: Air bubbles were caused by not having the generator deep enough within the medium. It is recommended that either a smaller generator be used or the volume of medium be increased.


Rodent embryo
PRODUCT APPLICATION REPORT
MATERIAL: Rodent embryo
VOLUME OF MATERIAL: 2 ml (All gen. were tested)
MEDIUM: Aqueous
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization for PCR work and DNA synthesis
PREVIOUS OR CURRENT METHOD: Hand pestel

EQUIPMENT USED: PRO200 Homogenizer  with 5nm, 7mm, and 1Omm generators
APPLICATION AND RESULTS: Excellent results achieved.


Salmon sperm
PRODUCT APPLICATION REPORT
MATERIAL: Salmon sperm
VOLUME OF MATERIAL: 5ml (7mm gen.) and 25 ml (20mm gen.)
MEDIUM: Blocking solution
VOLUME OF MEDIUM: Not reported
PROCESS: Liquefaction of sperm for DNA analysis
PREVIOUS OR CURRENT METHOD: 23-guage-liquefaction needle
EQUIPMENT USED: PRO200 Homogenizer  with 7mm and 10mm generators
APPLICATION AND RESULTS: Desired liquefaction results in 2-3
minutes.


Sea Weed
PRODUCT APPLICATION REPORT
MATERIAL: Sea Weed
VOLUME OF MATERIAL: 20 ml
MEDIUM: Not Reported
VOLUME OF MEDIUM: Not Reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Polytron PT1200 (did not homogenize sea weed - material wrapped around generator)
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator.
APPLICATION AND RESULTS: PRO200 Homogenizer with l0mm generator worked successfully. It out performed PT1200


Silica
PRODUCT APPLICATION REPORT
MATERIAL: Silica gel
VOLUME OF MATERIAL: 400 ml
MEDIUM: Aqueous
VOLUME OF MEDIUM: Not reported
PROCESS: Dispersion
PREVIOUS OR CURRENT METHOD: Blender
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer  with 20mm generator
APPLICATION AND RESULTS: successful.


Sediment
PRODUCT APPLICATION REPORT
MATERIAL: Sediment
VOLUME OF MATERIAL: Not reported
MEDIUM: Sediment water
VOLUME OF MEDIUM: Not reported
PROCESS: Not reported
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO250 Homogenizer
APPLICATION AND RESULTS: Successfully homogenized
Link DOI: http://dx.doi.org/10.1021/es203528v



Silicone
PRODUCT APPLICATION REPORT
MATERIAL: Silicone
VOLUME OF MATERIAL: 5 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Not reported
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: The PRO200 Homogenizer with 7mm generator produced
excellent results.


Skeletal muscle (human)
PRODUCT APPLICATION REPORT

MATERIAL: Skeletal muscle (human)
VOLUME OF MATERIAL: not reported
MEDIUM: lysis buffer
VOLUME OF MEDIUM: Not reported
PROCESS: "Homogenized for 20 strokes"
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200
APPLICATION AND RESULTS: Excellent results.
Link DOI: http://dx.doi.org/10.1016/j.jprot.2012.03.024


Skeletal muscle fat and liver powder
PRODUCT APPLICATION REPORT
MATERIAL: Skeletal muscle, fat, and liver powder
VOLUME OF MATERIAL: 1 g
MEDIUM: GTC buffer or phosphate buffer
VOLUME OF MEDIUM: 10 ml
PROCESS: RNA extraction through homogenized suspension
PREVIOUS OR CURRENT METHOD: Brinkmannn Polytron
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: Worked very well and saved time and
effort.


Snails
PRODUCT APPLICATION REPORT
MATERIAL: Snails
VOLUME OF MATERIAL: 5 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer with 7mm saw tooth generator
APPLICATION AND RESULTS: PRO200 Homogenizer with 7mm saw tooth generator was able to successfully homogenize the mini snails and their
shells completely.


Skin fibroblasts
PRODUCT APPLICATION REPORT
MATERIAL: Skin fibroblasts
VOLUME OF MATERIAL: 5 ml (5mm gen.) 50 ml (10mm gen.)
MEDIUM: Salt buffer
VOLUME OF MEDIUM: Not reported
PROCESS: Complete homogenization
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO200 Homogenizer with 5mm and 10mm generators
APPLICATION AND RESULTS: Achieved desired results successfully


Soil
PRODUCT APPLICATION REPORT
MATERIAL: Soil
VOLUME OF MATERIAL: 200 ml
MEDIUM: None
VOLUME OF MEDIUM: None
PROCESS: Homogenization of dry soil
PREVIOUS OR CURRENT METHOD: Meat grinder
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300 D homogenizer with blade and sealed
chamber assembly
APPLICATION AND RESULTS: Sample ground up into fine powder


Soybean cotyledon
PRODUCT APPLICATION REPORT
MATERIAL: Soybean cotyledon
VOLUME OF MATERIAL: 100 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Grinding
PREVIOUS OR CURRENT METHOD: Blender
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Worked well.


Soybean cotyledons
PRODUCT APPLICATION REPORT
MATERIAL: Soybean cotyledons
VOLUME OF MATERIAL: 50 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Complete homogenization


Soybean plant - various parts
PRODUCT APPLICATION REPORT
MATERIAL: Soybean plant
VOLUME OF MATERIAL: 5 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Rupture of samples
PREVIOUS OR CURRENT METHOD: Mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer with 7mm and 10mm generators
APPLICATION AND RESULTS: Successful application


Spleen
PRODUCT APPLICATION REPORT
MATERIAL: Spleen
VOLUME OF MATERIAL: 10 ml
MEDIUM: Buffer
VOLUME OF MEDIUM: 40 ml
PROCESS: Spleen disruption
PREVIOUS OR CURRENT METHOD: Tekmar Tissue-Mizer
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Good results.


Spore strips
PRODUCT APPLICATION REPORT
MATERIAL: Spore strips
VOLUME OF MATERIAL: 1 strip
MEDIUM: Tryptic Soy broth
VOLUME OF MEDIUM: 20 ml
PROCESS: Break up to recover bacteria
PREVIOUS OR CURRENT METHOD: Mortar and pestle and vortex stirrer
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Broken up easily. Increased cell
disruption achieve through longer homogenization.


Sturgeon barbles
PRODUCT APPLICATION REPORT
MATERIAL: Sturgeon barbles
VOLUME OF MATERIAL: 4 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Mitochondria extraction
PREVIOUS OR CURRENT METHOD: Mortar and pestle
EQUIPMENT USED: PRO200 with 7mm generator
APPLICATION AND RESULTS: 7mm saw tooth achieved desired results in
five minutes. To make sure that generated heat would not harm
sample, used ice bath.


Ticks
PRODUCT APPLICATION REPORT
MATERIAL: Ticks
VOLUME OF MATERIAL: 2 ticks
MEDIUM: PBS/PRS
VOLUME OF MEDIUM: 50 ml
PROCESS: Obtain 100% homogenization of the ticks.
PREVIOUS OR CURRENT METHOD: Manual homogenization
EQUIPMENT USED: PRO200 Homogenizer with 5mm generator
APPLICATION AND RESULTS: Homogenized ticks without any problems. System worked very well.


Tibialis anterior muscle
PRODUCT APPLICATION REPORT
MATERIAL: Tibialis anterior muscle
VOLUME OF MATERIAL: 20 mg
MEDIUM: potassium phosphate buffer (50 mM K 2 HPO 4 /KH 2 PO 4 , 1 mM EDTA, 0.1 mM DTT, pH 7.4) supplemented
with protease inhibitors
VOLUME OF MEDIUM:
PROCESS: Homogenized
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO Homogenizer 
APPLICATION AND RESULTS:

DOI:



http://dx.doi.org/10.1371/journal.pone.0081879




Tissue
PRODUCT APPLICATION REPORT
MATERIAL: Tissue
VOLUME OF MATERIAL: .25 ml
MEDIUM: GTI
VOLUME OF MEDIUM: .5 ml
PROCESS: Homogenize tissue to isolate RNA
PREVIOUS OR CURRENT METHOD: Brinkmannn instruments
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: Results were extremely successful


Tissue
PRODUCT APPLICATION REPORT
MATERIAL: Tissue
VOLUME OF MATERIAL: 10 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: 25 ml
PROCESS: Homogenize tissue with no degradation of RNA
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Excellent results


Tumor
PRODUCT APPLICATION REPORT
MATERIAL: Tumor
VOLUME OF MATERIAL: Not reported
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Not reported
PREVIOUS OR CURRENT METHOD: Break down tumor without disrupting cells
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: Successfully broke down the tumor without
disrupting cells.


Vanilla Callus tissue culture
PRODUCT APPLICATION REPORT
MATERIAL: Vanilla Callus tissue culture
VOLUME OF MATERIAL: 1/8"-1/4"
MEDIUM: Water
VOLUME OF MEDIUM: 2-10 ml
PROCESS: Complete homogenization
PREVIOUS OR CURRENT METHOD: Mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: Successfully broke the sample down with
the 7mm generator.


Various creams
PRODUCT APPLICATION REPORT
MATERIAL: Various creams
VOLUME OF MATERIAL: 100-250 ml
MEDIUM: None
VOLUME OF MEDIUM: 250-500 ml
PROCESS: Emulsion with even disbursement
PREVIOUS OR CURRENT METHOD: Brinkmannn, IKA, and other various
mixers
EQUIPMENT USED: PRO250 Homogenizer (w/ stand) or PRO300 D Homogenizer with a blade and
sealed chamber assembly
APPLICATION AND RESULTS: Complete emulsification and disbursement
with no lumps through blade system. Replaces other methods as best
application.


Various Eye Parts
PRODUCT APPLICATION REPORT
MATERIAL: Various eye parts
VOLUME OF MATERIAL: 5 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Break down tissue to extract cells
PREVIOUS OR CURRENT METHOD: None
EQUIPMENT USED: PRO200 Homogenizer with 7mm generator
APPLICATION AND RESULTS: Worked well


Virus
PRODUCT APPLICATION REPORT
MATERIAL: Virus
VOLUME OF MATERIAL: 25 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Extraction of virus for electrophoresis assay
PREVIOUS OR CURRENT METHOD: Hand turned mixer
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: Excellent homogenization of sample.


Waste bacteria
PRODUCT APPLICATION REPORT

MATERIAL: Waste bacteria
VOLUME OF MATERIAL: .05 ml (5mm gen.) and 50 ml (10mm gen.)
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Break down of bacteria through homogenization
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer with 5mm and 10mm generators
APPLICATION AND RESULTS: Products proved to be successful


Water oil mix
PRODUCT APPLICATION REPORT
MATERIAL: Water oil mix (olive oil)
VOLUME OF MATERIAL:
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS:
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO200 Homogenizer 
APPLICATION AND RESULTS:

DOI:
Products proved to be successful

http://dx/doi.org/10.1039/C3FO60380F


Wheat flour
PRODUCT APPLICATION REPORT
MATERIAL: Wheat flour
VOLUME OF MATERIAL: water flour ratio 1.7
MEDIUM: water
VOLUME OF MEDIUM: water flour ratio 1.7
PROCESS: 6000 rpm, 2 minutes
PREVIOUS OR CURRENT METHOD: Not reported
EQUIPMENT USED: PRO350PC Homogenizer with 37mm PRO Quick Connect Generator
APPLICATION AND RESULTS: Successfully homogenized and standard was established for bench-scale high-shear wet-milling test
Link DOI: http://dx.doi.org/10.1016/j.jfoodeng.2012.02.018


Wheat leaves
PRODUCT APPLICATION REPORT
MATERIAL: Wheat leaves
VOLUME OF MATERIAL: 25 ml
MEDIUM: Extraction buffer
VOLUME OF MEDIUM: 25 ml
PROCESS: Fine grinding of plant tissue for DNA analysis
PREVIOUS OR CURRENT METHOD: Mortar and pestle after freezing
with liquid nitrogen
EQUIPMENT USED: PRO200 Homogenizer with 10mm generator
APPLICATION AND RESULTS: DNA was broken down into short pieces
that could not analyze. Big chunks were needed for proper examination.
RECOMMENDATIONS: Perhaps a blade system can give the larger sample sizes that are needed for this type of application.


Whole cornea
PRODUCT APPLICATION REPORT
MATERIAL: Whole cornea
VOLUME OF MATERIAL: 5 ml
MEDIUM: Not reported
VOLUME OF MEDIUM: Not reported
PROCESS: Homogenization
PREVIOUS OR CURRENT METHOD: Mortar and pestle
EQUIPMENT USED: PRO200 Homogenizer with 7mm and 10mm generator
APPLICATION AND RESULTS: The 7mm generator was too small to handle
large cornea chunks. However, the 10mm was perfect for the
application.


Wild tomato vines
PRODUCT APPLICATION REPORT
MATERIAL: Wild tomato vines
VOLUME OF MATERIAL: 3g (5mm gen.) and 6 g (7mm gen.)
MEDIUM: Aqueous buffer
VOLUME OF MEDIUM: 5 ml (5mm gen.) and 10 ml (7mm gen.)
PROCESS: Dispersion and homogenization
PREVIOUS OR CURRENT METHOD: Virtishear mixer
EQUIPMENT USED: PRO200 Homogenizer with 5mm and 10mm generators
APPLICATION AND RESULTS: Worked excellent. 


Yeast cells

Zebrafish (embryo-larval)
PRODUCT APPLICATION REPORT
MATERIAL: Zebrafish (embryo-larval)
VOLUME OF MATERIAL:
MEDIUM: Trizol reagent
VOLUME OF MEDIUM:
PROCESS: Isolation of RNA
PREVIOUS OR CURRENT METHOD:
EQUIPMENT USED: PRO200 Homogenizer
APPLICATION AND RESULTS:

DOI:
Thirty embryos per group were homogenized with a Bio-Gen PRO200 homogenizer for the isolation of total RNA, using Trizol reagent.

http://dx.doi.org/10.1016/j.chemosphere.2013.10.014






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