Potter-Elvehjem Grinding Chambers and Pestles vs. PRO Scientific Homogenizer
With a Tissue Grinder Potter-Elvehjem, the PTFE pestle is attached to a stainless steel shaft and homogenization occurs as the sample and buffer are forced through the cylindrical portion of the mortar as the pestle rotates downward. Generally this is a hand-held process since all movement is done by hand. Using a Potter-Elvehjem homogenizer with a PTFE pestle is a viable option for disrupting cells. However, it is not very efficient at homogenizing solid tissue.
However, you need not be limited to using only a Tissue Grinder Potter-Elvehjem with your soft tissue samples, such as liver or brain. A PRO Scientific rotor-stator homogenizer can be an effective means of homogenizing soft tissues. When using a rotor-stator homogenizer, like PRO Scientific’s homogenizer, it is important to select a generator probe that provides a low sheer number. With that generator probe selected, most homogenization will take place in a matter of seconds, so heat production is not a concern.
Examples of PRO Scientific Homogenizers being used to homogenize soft tissue samples: